Engineering of therapeutic polypeptides through chemical synthesis: Early lessons from human parathyroid hormone and analogues Journal Article
| Authors: | Dong, S.; Shang, S.; Li, J.; Tan, Z.; Dean, T.; Maeda, A.; Gardella, T. J.; Danishefsky, S. J. |
| Article Title: | Engineering of therapeutic polypeptides through chemical synthesis: Early lessons from human parathyroid hormone and analogues |
| Abstract: | Application of chemical synthesis to gain access to high purity hPTH as well as more stable analogues was accomplished through a menu of extended NCL followed by metal free dethiylation. © 2012 American Chemical Society. |
| Keywords: | signal transduction; controlled study; protein expression; nonhuman; binding affinity; chemical analysis; protein conformation; mouse; animals; mice; animal experiment; animal model; body weight; calcium; substitution reaction; shelf life; mice, inbred c57bl; biosynthesis; chemistry; transcription regulation; amino acid sequence; amino terminal sequence; biological activity; peptides; chemical structure; molecular structure; high performance liquid chromatography; cyclization; parathyroid hormone; injections, subcutaneous; oxidation; synthesis; synthesis (chemical); chemical synthesis; cysteine; peptide synthesis; physical chemistry; bioavailability; chemical modification; polypeptide; degradation; solid phase synthesis; hek293 cells; glomerulus filtration; purification; protein engineering; dethiylation; high purity; parathyroid hormones; chemical engineering |
| Journal Title: | Journal of the American Chemical Society |
| Volume: | 134 |
| Issue: | 36 |
| ISSN: | 0002-7863 |
| Publisher: | American Chemical Society |
| Date Published: | 2012-09-12 |
| Start Page: | 15122 |
| End Page: | 15129 |
| Language: | English |
| DOI: | 10.1021/ja306637u |
| PROVIDER: | scopus |
| PUBMED: | 22891619 |
| PMCID: | PMC3462362 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 October 2012" - "CODEN: JACSA" - "Source: Scopus" - PMCID available 9/12/2013 |
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