Comparison of the Luminex xTAG RVP fast assay and the Idaho technology FilmArray RP assay for detection of respiratory viruses in pediatric patients at a cancer hospital Journal Article

   

Authors:	Babady, N. E.; Mead, P.; Stiles, J.; Brennan, C.; Li, H.; Shuptar, S.; Stratton, C. W.; Tang, Y. W.; Kamboj, M.
Article Title:	Comparison of the Luminex xTAG RVP fast assay and the Idaho technology FilmArray RP assay for detection of respiratory viruses in pediatric patients at a cancer hospital
Abstract:	Respiratory viruses are increasingly recognized as serious causes of morbidity and mortality in immunocompromised patients. The rapid and sensitive detection of respiratory viruses is essential for the early diagnosis and administration of appropriate antiviral therapy, as well as for the effective implementation of infection control measures. We compared the performance of two commercial assays, xTAG RVP Fast (Luminex Diagnostics, Toronto, Canada) and FilmArray RVP (FA RVP; Idaho Technology, Salt Lake City, UT), in pediatric patients at Memorial Sloan-Kettering Cancer Center. These assays detect the following viruses: respiratory syncytial virus; influenza A and B viruses; parainfluenza viruses 1, 2, 3, and 4; human metapneumovirus; adenovirus; enterovirus-rhinovirus; coronaviruses NL63, HKU1, 229E, and OC43; and bocavirus. We tested a total of 358 respiratory specimens from 173 pediatric patients previously tested by direct fluorescence assay (DFA) and viral culture. The overall detection rate (number of positive specimens/total specimens) for viruses tested by all methods was 24% for DFA/culture, 45% for xTAG RVP Fast, and 51% for FA RVP. The agreement between the two multiplex assays was 84.5%, and the difference in detection rate was statistically significant (P < 0.0001). Overall, the FA RVP assay was more sensitive than the xTAG RVP Fast assay and had a turnaround time of approximately 1 h. The sensitivity, simplicity, and random-access platform make FA RVP an excellent choice for laboratory on-demand service with low to medium volume. Copyright © 2012, American Society for Microbiology. All Rights Reserved.
Keywords:	adolescent; adult; child; controlled study; child, preschool; young adult; major clinical study; nonhuman; validation process; sensitivity and specificity; neoplasms; virology; cancer center; infant; laboratory test; cancer care facilities; intermethod comparison; fluorescence analysis; virus strain; virus diseases; respiratory syncytial pneumovirus; respiratory virus; virus detection; upper respiratory tract infection; adenovirus; molecular diagnostic techniques; child health care; viruses; respiratory tract infections; virus culture; human metapneumovirus; influenza a; bocavirus; enterovirus; human coronavirus nl63; influenza b; laboratory device; parainfluenza virus 1; parainfluenza virus 2; parainfluenza virus 3; parainfluenza virus 4; respiratory viral panel assay; rhinovirus; virus examination; clinical laboratory techniques
Journal Title:	Journal of Clinical Microbiology
Volume:	50
Issue:	7
ISSN:	0095-1137
Publisher:	American Society for Microbiology
Date Published:	2012-07-01
Start Page:	2282
End Page:	2288
Language:	English
DOI:	10.1128/jcm.06186-11
PROVIDER:	scopus
PUBMED:	22518855
PMCID:	PMC3405573
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84862749719&partnerID=40&md5=0931d4e43388ea4a1632d38ded45bfe9
Notes:	--- - "Export Date: 1 August 2012" - "CODEN: JCMID" - "Source: Scopus"

https://synapse.mskcc.org/synapse/works/has_related_data_chk/31797