Comparative performance of the Luminex NxTAG Respiratory Pathogen Panel, GenMark eSensor Respiratory Viral Panel, and BioFire FilmArray Respiratory Panel Journal Article
| Authors: | Popowitch, E. B.; Kaplan, S.; Wu, Z.; Tang, Y. W.; Miller, M. B. |
| Article Title: | Comparative performance of the Luminex NxTAG Respiratory Pathogen Panel, GenMark eSensor Respiratory Viral Panel, and BioFire FilmArray Respiratory Panel |
| Abstract: | This study compares three of the most inclusive and widely used panels for respiratory syndromic testing in the United States, namely, Luminex NxTAG Respiratory Pathogen Panel (RPP), BioFire FilmArray Respiratory Panel (RP), and GenMark eSensor Respiratory Viral Panel (RVP). We compared the three assays using nasopharyngeal swab samples (n = 350) collected from symptomatic patients (n = 329) in the pre-coronavirus disease 2019 (COVID-19) era. There was no significant difference in the overall accuracies of BioFire and Luminex assays (P = 0.6171); however, significant differences were found between BioFire and GenMark (P = 0.0003) and between GenMark and Luminex (P = 0.0009). The positive percent agreement of the BioFire RP assay was 94.1%, compared to 97.3% for GenMark RVP and 96.5% for Luminex RPP. Overall negative percent agreement values were high for all three assays, i.e., 99.9% for BioFire and Luminex and 99.5% for GenMark. The three assays were equivalent for adenovirus, human metapneumovirus, influenza A, and respiratory syncytial virus. Increased false-positive results were seen with BioFire for the endemic coronaviruses and with GenMark for influenza B and the parainfluenza viruses. © 2022 Popowitch et al. |
| Keywords: | adult; aged; major clinical study; genetics; united states; influenza; false positive result; virus; respiratory tract infection; influenza, human; adenoviridae; molecular diagnosis; molecular diagnostic techniques; viruses; respiratory tract infections; procedures; endemic disease; human metapneumovirus; influenza a virus; humans; human; article; human respiratory syncytial virus; genmark; luminex; coronavirus disease 2019; covid-19; biofire; syndromic testing; nasopharyngeal swab; respiratory pathogen panels; respiratory viral panels; influenza b virus; paramyxovirinae |
| Journal Title: | Microbiology Spectrum |
| Volume: | 10 |
| Issue: | 4 |
| ISSN: | 2165-0497 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2022-07-01 |
| Start Page: | e0124822 |
| Language: | English |
| DOI: | 10.1128/spectrum.01248-22 |
| PUBMED: | 35766513 |
| PROVIDER: | scopus |
| PMCID: | PMC9431521 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 3 October 2022 -- Source: Scopus |
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