Defining the Mer1 and Nam8 meiotic splicing regulons by cDNA rescue Journal Article
| Authors: | Qiu, Z. R.; Schwer, B.; Shuman, S. |
| Article Title: | Defining the Mer1 and Nam8 meiotic splicing regulons by cDNA rescue |
| Abstract: | Meiosis-specific pre-mRNA splicing in budding yeast embraces multiple pre-mRNA targets grouped inTo regulons defined by their genetic requirements for vegetatively optional splicing factors (e.g. splicing enhancer Mer1 and the U1 snRNP subunit Nam8) or snRNA modifications (trimethylguanosine caps). Here we genetically demarcate a complete meiotic splicing regulon by the criterion of cDNA bypass of the requirement for the governing splicing regulators to execute sporulation. We thereby show that the Mer1 and Nam8 regulons embrace four essential pre-mRNAs: MER2 MER3 SPO22 and AMA1. Whereas Nam8 also regulates PCH2 splicing PCH2 cDNA is not needed for sporulation by nam8Δ diploids. Our results show that there are no essential intron-containing RNAs missing from the known roster of Mer1 and Nam8 targets. Nam8 is composed of three RRM domains flanked by N-terminal leader and C-terminal tail segments. We find that the RRM2 and RRM3 domains and their putative RNA-binding sites are essential for yeast sporulation whereas the leader tail and RRM1 modules are not. Copyright © 2011 RNA Society. |
| Keywords: | controlled study; unclassified drug; gene mutation; mutation; nonhuman; protein domain; protein function; meiosis; cell cycle proteins; chromosomal proteins, non-histone; gene expression; carboxy terminal sequence; protein targeting; intron; introns; rna; rna-binding proteins; amino acid sequence; molecular sequence data; amino terminal sequence; saccharomyces cerevisiae; binding site; yeast; saccharomyces cerevisiae proteins; saccharomycetales; dna helicases; fungal protein; rna binding; gene expression regulation, fungal; rna splicing; complementary dna; rna precursors; diploidy; dna, complementary; messenger rna precursor; polypeptide; nam8 protein; sporogenesis; regulon; ama1; mer2; mer3; spo22; ribonucleoproteins, small nuclear; rna, small nuclear; mer2 protein; ama1 protein; mer1 protein; mer3 protein; pch2 protein; rrm1 protein; rrm2 protein; rrm3 protein; spo22 protein; ribonucleoprotein, u1 small nuclear |
| Journal Title: | RNA |
| Volume: | 17 |
| Issue: | 9 |
| ISSN: | 1355-8382 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 2011-09-01 |
| Start Page: | 1648 |
| End Page: | 1654 |
| Language: | English |
| DOI: | 10.1261/rna.2792011 |
| PUBMED: | 21788335 |
| PROVIDER: | scopus |
| PMCID: | PMC3162330 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 4 June 2012" - "CODEN: RNARF" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work