Probing the stability of nonglycosylated wild-type erythropoietin protein via reiterative alanine ligations Journal Article
| Authors: | Brailsford, J. A.; Danishefsky, S. J. |
| Article Title: | Probing the stability of nonglycosylated wild-type erythropoietin protein via reiterative alanine ligations |
| Abstract: | Nonglycosylated erythropoietin bearing acetamidomethyl protecting groups at the cysteine residues has been synthesized via chemical methods. Alanine ligation was used to assemble four peptide fragments, themselves prepared by solid phase peptide synthesis. This work outlines a route for the synthesis of homogeneous glycosylated erythropoietin. |
| Keywords: | controlled study; erythropoietin; protein stability; wild type; amino acid sequence; molecular sequence data; peptide fragments; peptide fragment; glycosylation; molecular structure; alanine; protein structure; protein structure, secondary; models, chemical; carbohydrate sequence; desulfurization; cysteine; peptide synthesis; oligosaccharides; solid phase synthesis; methyl group; protein glycosylation; solid phase peptide synthesis |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 109 |
| Issue: | 19 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2012-05-08 |
| Start Page: | 7196 |
| End Page: | 7201 |
| Language: | English |
| DOI: | 10.1073/pnas.1202762109 |
| PROVIDER: | scopus |
| PMCID: | PMC3358843 |
| PUBMED: | 22499784 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 4 June 2012" - "CODEN: PNASA" - "Source: Scopus" |
