| Abstract: |
FoxG1 (formerly BF-1) encodes a transcription factor that regulates neurogenesis in the embryonic telencephalon. The current study suggests that FoxG1 also regulates neurogenesis in the postnatal hippocampus. FoxG1 continues to be strongly expressed in areas of known postnatal neurogenesis, including the subventricular zone of the lateral ventricle and the dentate gyrus (DG) of the hippocampus. Remarkably, FoxG1 +/- mice have a 60% decrease in the total number of hippocampal dentate granule cells that is related to a loss of DG neurogenesis. Comparison of acute and chronic BrdU labeling, and PSA-NCAM staining suggests that the stage at which this loss of neurogenesis occurs progresses with age. Juvenile mice FoxG1 +/- primarily show failed apparent survival of postnatally born DG neurons, whereas adult FoxG1 +/- mice also show impairment of proliferation and initial DG neuron differentiation. Consistent with this process predominantly affecting postnatal hippocampal neurogenesis, BrdU pulses at embryonic days 16, 17, and 18 labels a higher percentage of DG cells in 6-week-old FoxG1 +/- mice than in littermate controls. In contrast to the marked effect of FoxG1 haploinsufficiency on postnatal hippocampal neurogenesis, postnatal neurogenesis of olfactory bulb interneurons is grossly unaffected. Behaviorally, FoxG1 +/- mice show hyperlocomotion and impaired habituation in the open field, and a severe deficit in contextual fear conditioning that are suggestive of impaired hippocampal function. Although mechanistic connections between FoxG1 haploinsufficiency and either failed postnatal DG neurogenesis or the behavioral deficits remain to be elucidated, these results present a new model system for impaired postnatal neurogenesis in the DG of adult mice. © 2006 Wiley-Liss, Inc. |
| Keywords: |
controlled study; unclassified drug; nonhuman; cell proliferation; forkhead transcription factors; mouse; phenotype; animals; mice; mice, knockout; animal tissue; cell survival; genetic predisposition to disease; gene expression; animal experiment; nerve tissue proteins; haplotypes; transcription factor; cell differentiation; neurons; mice, inbred c57bl; gene expression regulation, developmental; regulatory mechanism; interneuron; granule cell; staining; stem cells; cell count; animals, newborn; memory disorder; disease models, animal; fear; transcription factor foxg1; nerve cell differentiation; nervous system development; memory disorders; brain function; locomotion; olfactory bulb; cell labeling; broxuridine; bromodeoxyuridine; nerve degeneration; perinatal period; hippocampus; telencephalon; dentate gyrus; brain nerve cell; conditioning; lateral brain ventricle; psa-ncam; hippocampal neurogenesis; bf1; contextual learning; habituation; open field test; avoidance learning; hyperkinesis
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