Apoptotic and cell cycle regulatory markers in uterine leiomyosarcoma Journal Article


Authors: Leiser, A. L.; Anderson, S. E.; Nonaka, D.; Chuai, S.; Olshen, A. B.; Chi, D. S.; Soslow, R. A.
Article Title: Apoptotic and cell cycle regulatory markers in uterine leiomyosarcoma
Abstract: Objectives. The primary aim of this study was to investigate the expression of apoptotic and cell cycle regulators p53, p21, p27, bax, and bcl-2 in uterine leiomyosarcoma in order to identify molecular pathways that possibly could be important in the development of leiomyosarcoma. A secondary aim was to examine if the apoptotic and cell cycle regulatory protein expression profile of uterine leiomyosarcoma is potentially useful for clinical prognostic purposes. Methods. A tissue microarray representing 36 uterine leiomyosarcomas and 19 uterine leiomyomas was created with 3 representative cores from each tumor. Immunohistochemical staining was performed for bcl-2, bax, p21, p27, and p53 using standard techniques. Staining was scored 0-12 for each marker, 0-3 being negative and 4-12 positive. Outcome analyses were performed only for leiomyosarcomas. First recurrence was determined from the time of initial diagnosis. Survival was determined from the time of initial diagnosis to last follow-up. Results. Associations were found between disease type (leiomyosarcoma vs. leiomyoma) and the positivity status of p21 (43% vs. 0%, P < 0.001), p53 (54% vs. 0%, P < 0.001), and bax (34% vs. 94%, P < 0.001). bcl-2-positive leiomyosarcoma was associated with a longer time to recurrence (P = 0.02) in a univariate analysis. In a multivariate analysis, tumor stage was the only independent significant prognostic factor (P = 0.002). Conclusion. The significant differential expression of apoptotic and cell cycle regulatory proteins in uterine leiomyosarcoma as compared to benign smooth muscle tumors suggests that pathways involving these proteins may be important in the development of malignant disease and, therefore, could be potential targets for molecular therapies. © 2005 Elsevier Inc. All rights reserved.
Keywords: immunohistochemistry; adult; cancer survival; controlled study; human tissue; protein expression; aged; middle aged; survival rate; human cell; major clinical study; follow up; cancer diagnosis; neoplasm staging; cell cycle proteins; cell cycle; protein bcl 2; apoptosis; tumor markers, biological; molecular dynamics; protein p53; protein p27; tissue microarray; leiomyosarcoma; protein p21; uterine neoplasms; uterus myoma; uterine leiomyosarcoma; p53; p21; bax; bcl-2; protein bax; leiomyoma
Journal Title: Gynecologic Oncology
Volume: 101
Issue: 1
ISSN: 0090-8258
Publisher: Elsevier Inc.  
Date Published: 2006-04-01
Start Page: 86
End Page: 91
Language: English
DOI: 10.1016/j.ygyno.2005.09.055
PUBMED: 16289259
PROVIDER: scopus
DOI/URL:
Notes: --- - "Cited By (since 1996): 16" - "Export Date: 4 June 2012" - "CODEN: GYNOA" - "Source: Scopus"
Altmetric Score
MSK Authors
  1. Daisuke Nonaka
    6 Nonaka
  2. Shaokun Chuai
    29 Chuai
  3. Aliza Leah Leiser
    8 Leiser
  4. Dennis S Chi
    496 Chi
  5. Adam B Olshen
    107 Olshen
  6. Robert Soslow
    643 Soslow