Multimodal microscopy of immune cells and melanoma for longitudinal studies Conference Paper
| Authors: | Entenberg, D.; Aranda, I.; Li, Y.; Toledo-Crow, R.; Schaer, D. |
| Title: | Multimodal microscopy of immune cells and melanoma for longitudinal studies |
| Conference Title: | Multimodal Biomedical Imaging |
| Abstract: | Interavital microscopy of cancer is a well established tool that provides direct visualization of the tumor cycle. It traditionally involves one of several strategies: invasive subcutaneous (SC) implantation of tumors followed by surgical opening of skin flaps for imaging, techniques utilizing skin fold chambers and implanted optical windows or intradermal injections under 200μm from the skin surface. All of these techniques allow the use of fluorescent proteins as markers for biologically significant constituents. However, observation methods utilizing skin-flaps, skin-fold chambers and optical windows are invasive and tend to alter the immune environment of the tissue and/or limit the duration of studies that can be performed. If implanted correctly, intradermally injected rumors can be minimally invasive, will not require biopsies or surgical intervention to observe and are accessible for direct transdermal imaging with a number of in vivo modalities. We present our work in the development of a small animal intravital microscopy workstation that allows the acquisition of different contrast imaging modalities: reflectance confocal, wide field epifluorescence, multiphoton and second harmonic generation (SHG). The images are acquired pair-wise simultaneously and sequentially in time. The aim of our instrumentation is to gather all information generated by the single probing beam via the reflected or back-scattered signal, SHG signal and various fluorescence signals. Additionally, we also present our development of a microscopic tissue navigation technique to mark, label and track sites of interest. This technique enables us to revisit sites periodically and record, with different imaging contrasts, their biological changes over time. |
| Keywords: | proteins; fluorescence; gfp; skin; immunology; tumors; surgery; navigation; cells; 2 photon; longitudinal melanoma study; multimodal intravital microscopy |
| Journal Title | Proceedings of SPIE |
| Volume: | 6081 |
| Conference Dates: | 2006 Jan 21-24 |
| Conference Location: | San Jose, CA |
| ISBN: | 0277-786X |
| Publisher: | SPIE |
| Date Published: | 2006-01-01 |
| Start Page: | 60810A |
| Language: | English |
| DOI: | 10.1117/12.646907 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - Proceedings of SPIE - The International Society for Optical Engineering - Proc SPIE Int Soc Opt Eng - "Conference code: 67204" - "Cited By (since 1996): 1" - "Export Date: 4 June 2012" - "CODEN: PSISD" - "Sponsors: SPIE" - 21 January 2006 through 24 January 2006 - "Source: Scopus" |
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20Li -
6Aranda
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