Identification of a new cancer/testis gene family, CT47, among expressed multicopy genes on the human X chromosome Journal Article
| Authors: | Chen, Y. T.; Iseli, C.; Venditti, C. A.; Old, L. J.; Simpson, A. J. G.; Jongeneel, C. V. |
| Article Title: | Identification of a new cancer/testis gene family, CT47, among expressed multicopy genes on the human X chromosome |
| Abstract: | Cancer/testis (CT) genes are normally expressed in germ cells only, yet are reactivated and expressed in some tumors. Of the approximately 40 CT genes or gene families identified to date, 20 are on the X chromosome and are present as multigene families, many with highly conserved members. This indicates that novel CT gene families may be identified by detecting duplicated expressed genes on chromosome X. By searching for transcript clusters that map to multiple locations on the chromosome, followed by in silico analysis of their gene expression profiles, we identified five novel gene families with testis-specific expression and >98% sequence identity among family members. The expression of these genes in normal tissues and various tumor cell lines and specimens was evaluated by qualitative and quantitative RT-PCR, and a novel CT gene family with at least 13 copies was identified on Xq24, designated as CT47. mRNA expression of CT47 was found mainly in the testes, with weak expression in the placenta. Brain tissue was the only positive somatic tissue tested, with an estimated CT47 transcript level 0.09% of that found in testis. Among the tumor specimens tested, CT47 expression was found in ∼15% of lung cancer and esophageal cancer specimens, but not in colorectal cancer or breast cancer. The putative CT47 protein consists of 288 amino acid residues, with a C-terminus rich in alanine and glutamic acid. The only species other than human in which a gene homologous to CT47 has been detected is the chimpanzee, with the predicted protein showing ∼80% identity in its carboxy terminal region. © 2005 Wiley-Liss, Inc. |
| Keywords: | human tissue; unclassified drug; human cell; neoplasms; colorectal cancer; proteins; gene; reverse transcription polymerase chain reaction; breast cancer; gene expression; gene expression profiling; carboxy terminal sequence; germ cell; lung cancer; gene product; cell line, tumor; gene activation; amino acid sequence; messenger rna; reverse transcriptase polymerase chain reaction; gene identification; nucleotide sequence; gene duplication; x chromosome; chromosomes, human, x; alanine; gene dosage; sequence homology; esophagus cancer; glutamic acid; testis; expressed sequence tags; gene location; chromosome map; multigene family; chromosome xp; genes, neoplasm; pan troglodytes; chimpanzee; protein ct47; ct47 gene |
| Journal Title: | Genes Chromosomes and Cancer |
| Volume: | 45 |
| Issue: | 4 |
| ISSN: | 1045-2257 |
| Publisher: | Wiley Periodicals, Inc |
| Date Published: | 2006-04-01 |
| Start Page: | 392 |
| End Page: | 400 |
| Language: | English |
| DOI: | 10.1002/gcc.20298 |
| PUBMED: | 16382448 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 13" - "Export Date: 4 June 2012" - "CODEN: GCCAE" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work