Authors: | Berrozpe, G.; Agosti, V.; Tucker, C.; Blanpain, C.; Manova, K.; Besmer, P. |
Article Title: | A distant upstream locus control region is critical for expression of the kit receptor gene in mast cells |
Abstract: | The Kit receptor tyrosine kinase functions in hematopoiesis, melanogenesis, and gametogenesis and in interstitial cells of Cajal. We previously identified two upstream hypersensitive site (HS) clusters in mast cells and melanocytes. Here we investigated the roles of these 5′ HS sequences in Kit expression using transgenic mice carrying Kit-GFP reporter constructs. In these mice there is close correspondence between Kit-GFP reporter and endogenous Kit gene expression in most tissues analyzed. Deletion analysis defined the 5′ upstream HS cluster region as critical for Kit expression in mast cells. Furthermore, chromatin immunoprecipitation analysis in mast cells showed that H3 and H4 histone hyperacetylation and RNA polymerase II recruitment within the Kit promoter and in the 5′ HS region were associated with Kit expression. Therefore, the 5′ upstream hypersensitivity sites appear to be critical components of locus control region-mediated Kit gene activation in mast cells. Copyright © 2006, American Society for Microbiology. All Rights Reserved. |
Keywords: | controlled study; gene cluster; promoter region; gene deletion; nonhuman; genetic analysis; protein function; protein analysis; animal cell; mouse; animals; mice; animal tissue; cerebellum; bone marrow cells; cells, cultured; stem cell factor; stem cell factor receptor; interstitial cell of cajal; proto-oncogene proteins c-kit; gene expression; melanocyte; green fluorescent protein; gene locus; protein tyrosine kinase; transgenic mouse; mus musculus; mice, transgenic; gene expression regulation; ovary; gene activation; recombinant fusion proteins; chromatin; chromatin immunoprecipitation; histone h3; hematopoiesis; dna flanking region; genes, reporter; nucleic acid conformation; rna polymerase ii; histones; testis; receptor gene; melanogenesis; acetylation; gene construct; histone h4; mast cell; mast cells; receptor protein; gametogenesis; regulator gene; locus control region; hypersensitive site |
Journal Title: | Molecular and Cellular Biology |
Volume: | 26 |
Issue: | 15 |
ISSN: | 0270-7306 |
Publisher: | American Society for Microbiology |
Date Published: | 2006-08-01 |
Start Page: | 5850 |
End Page: | 5860 |
Language: | English |
DOI: | 10.1128/mcb.01854-05 |
PUBMED: | 16847336 |
PROVIDER: | scopus |
PMCID: | PMC1592758 |
DOI/URL: | |
Notes: | --- - "Cited By (since 1996): 12" - "Export Date: 4 June 2012" - "CODEN: MCEBD" - "Source: Scopus" |