Role of the gut endoderm in relaying left-right patterning in mice Journal Article

Authors: Viotti, M.; Niu, L.; Shi, S. H.; Hadjantonakis, A. K.
Article Title: Role of the gut endoderm in relaying left-right patterning in mice
Abstract: Establishment of left-right (LR) asymmetry occurs after gastrulation commences and utilizes a conserved cascade of events. In the mouse, LR symmetry is broken at a midline structure, the node, and involves signal relay to the lateral plate, where it results in asymmetric organ morphogenesis. How information transmits from the node to the distantly situated lateral plate remains unclear. Noting that embryos lacking Sox17 exhibit defects in both gut endoderm formation and LR patterning, we investigated a potential connection between these two processes. We observed an endoderm-specific absence of the critical gap junction component, Connexin43 (Cx43), in Sox17 mutants. Iontophoretic dye injection experiments revealed planar gap junction coupling across the gut endoderm in wild-type but not Sox17 mutant embryos. They also revealed uncoupling of left and right sides of the gut endoderm in an isolated domain of gap junction intercellular communication at the midline, which in principle could function as a barrier to communication between the left and right sides of the embryo. The role for gap junction communication in LR patterning was confirmed by pharmacological inhibition, which molecularly recapitulated the mutant phenotype. Collectively, our data demonstrate that Cx43-mediated communication across gap junctions within the gut endoderm serves as a mechanism for information relay between node and lateral plate in a process that is critical for the establishment of LR asymmetry in mice. © 2012 Viotti et al.
Keywords: signal transduction; controlled study; genetics; nonhuman; methodology; protein localization; mouse; phenotype; animal; metabolism; animals; mice; mus; gene expression profiling; embryo; green fluorescent protein; animal experiment; embryo development; embryo pattern formation; morphogenesis; institute for cancer research mouse; pathology; mice, inbred icr; wild type; physiology; animal embryo; gene expression regulation; prenatal development; gene expression regulation, developmental; tissue distribution; transgene; drug derivative; green fluorescent proteins; embryo, mammalian; cell junction; image processing, computer-assisted; image processing; gastrointestinal tract; cell communication; body patterning; transgenes; embryonic development; endoderm; mutant; transcription factor sox; biotin; transcription factor sox17; connexin 43; gap junction; gap junctions; hmgb proteins; gut endoderm; iontophoresis; left right patterning; gja1 protein, mouse; high mobility group b protein; neurobiotin; sox17 protein, mouse; soxf transcription factors
Journal Title: PLoS Biology
Volume: 10
Issue: 3
ISSN: 1544-9173
Publisher: Public Library of Science  
Date Published: 2012-03-01
Start Page: e1001276
Language: English
DOI: 10.1371/journal.pbio.1001276
PROVIDER: scopus
PMCID: PMC3295824
PUBMED: 22412348
Notes: --- - "Export Date: 1 May 2012" - "CODEN: PBLIB" - "Source: Scopus"
Citation Impact
MSK Authors
  1. Lei Niu
    3 Niu
  2. Song-Hai Shi
    52 Shi
  3. Manuel Viotti
    8 Viotti