FOXR2 activation is not exclusive of CNS neuroblastoma Journal Article
| Authors: | Siskar, A. N.; Hanzlik, E.; Cardenas, M. F.; Eldomery, M. K.; Pinto, S.; Tinkle, C. L.; Zhang, Q. Y.; Li, X. Y.; Lin, T.; Dhanda, S. K.; Reis, G.; Li, D.; Raghavan, R.; Vortmeyer, A.; Karajannis, M. A.; Robinson, G. W.; Onar-Thomas, A.; Blackburn, P. R.; Wheeler, D. A.; Chiang, J. S. |
| Article Title: | FOXR2 activation is not exclusive of CNS neuroblastoma |
| Abstract: | Background FOXR2 activation is regarded as pathognomonic for CNS neuroblastoma (NB). However, a comprehensive understanding of the landscape for CNS tumors exhibiting FOXR2 activation is lacking.Methods Histopathologic, molecular, imaging, and clinical data of 42 CNS tumors with FOXR2 overexpression identified through screening institutional datasets and published institutional cases were analyzed.Results Among the 42 tumors, 21 (50.0%) were high-grade gliomas (HGGs), and 18 (42.9%) were embryonal tumors. The HGGs included ten H3 K27M-mutant diffuse midline gliomas (DMGs) and eight radiation-associated tumors. The embryonal tumors included 11 CNS NBs and six pineoblastomas (PBs). FOXR2 expression was similar between CNS NB and other tumor types (P = 0.82). HGGs with FOXR2 overexpression, unlike NBs and PBs, displayed diverse concomitant genetic alterations. The most common mechanisms of FOXR2 activation involved structural alterations causing promoter donation and enhancer hijacking from active genes essential for brain development, followed by alternative promoter activation or truncated LINE-1 retrotransposition. The preferential activation mechanism varied by tumor type. All but two aberrant FOXR2 transcripts incorporated non-canonical, non-coding exons. Gene set enrichment analysis demonstrated shared downstream effects of FOXR2 activation at the epigenome and transcriptome levels across tumor types. DMGs and PBs with FOXR2 overexpression were aggressive, with 0% 2-year overall survival, whereas CNS NBs responded well to combined chemotherapy and radiation.Conclusions CNS tumors with FOXR2 overexpression manifest significant histological, molecular, imaging, and clinical diversity. While HGGs and PBs with FOXR2 overexpression demonstrated inferior prognosis, CNS NBs showed favorable outcomes. Integrating histologic and molecular diagnostic approaches is imperative for accurate prognostication and optimal therapeutic decision-making. |
| Keywords: | methylation; classification; oncogene; neurooncology; response assessment; patient outcomes; line-1 retrotransposition; alternative promoter; cns tumors with foxr2 overexpression; promoter donation and enhancer hijacking; glioma recommendations |
| Journal Title: | Neuro-Oncology |
| ISSN: | 1522-8517 |
| Publisher: | Oxford University Press |
| Publication status: | Online ahead of print |
| Date Published: | 2025-01-01 |
| Online Publication Date: | 2025-01-01 |
| Language: | English |
| ACCESSION: | WOS:001467672700001 |
| DOI: | 10.1093/neuonc/noaf076 |
| PROVIDER: | wos |
| Notes: | Article; Early Access -- Source: Wos |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work