IL-2Rα is dispensable for murine B cell development and humoral response Journal Article
| Authors: | Chowdhury, P.; Belcheva, K. T.; Smolkin, R. M.; Cols, M.; Fontenot, J. D.; Yewdell, W. T.; Chaudhuri, J. |
| Article Title: | IL-2Rα is dispensable for murine B cell development and humoral response |
| Abstract: | The cytokine IL-2 plays a pivotal role in the immune system, specifically in the proliferation of T, B, and NK cells. The alpha subunit of the IL-2 receptor, IL-2Rα (CD25), is known to regulate the expansion and differentiation of T lymphocytes. CD25 is also expressed in developing B cells; however, its B cell intrinsic role remains undefined. We generated a mouse model with a B cell-specific deletion of CD25 to ascertain its role in B cell development and function. Unexpectedly, we found that the loss of CD25 had no impact on B cell development, homeostasis, or immune response to model antigens. Additionally, while CD25 expression was upregulated in activated splenic B cells, its absence did not affect class switch recombination in vitro or in vivo. We conclude that in contrast to its critical role in T cell differentiation and function, and despite its expression in developing and activated B cells, CD25 does not have any significant role in B cell development and adaptive immune functions. © The Author(s) 2025. |
| Keywords: | controlled study; protein expression; human cell; genetics; nonhuman; flow cytometry; cd8+ t lymphocyte; cell proliferation; t lymphocyte; animal cell; mouse; animal; cytology; metabolism; animals; mice; mice, knockout; animal tissue; interleukin 2; gene expression; cell maturation; dendritic cell; interleukin 10; animal experiment; animal model; cell differentiation; enzyme linked immunosorbent assay; mice, inbred c57bl; b lymphocyte; c57bl mouse; b-lymphocytes; granzyme b; t lymphocyte receptor; regulatory t lymphocyte; immunology; lymphocyte activation; class switch recombination; immune response; gamma interferon; cd4+ t lymphocyte; real time polymerase chain reaction; th17 cell; cd4 antigen; immunoglobulin class switching; adaptive immunity; autoantibody; humoral immunity; fluorescence activated cell sorting; knockout mouse; peripheral blood mononuclear cell; interleukin 2 receptor alpha; interleukin-2 receptor alpha subunit; rna sequence; cd25; immunity, humoral; il2ra protein, mouse; human; article; germinal centers; b cell development |
| Journal Title: | Journal of Immunology |
| Volume: | 214 |
| Issue: | 4 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 2025-04-01 |
| Start Page: | 694 |
| End Page: | 702 |
| Language: | English |
| DOI: | 10.1093/jimmun/vkae045 |
| PUBMED: | 40096628 |
| PROVIDER: | scopus |
| PMCID: | PMC12041769 |
| DOI/URL: | |
| Notes: | Article -- MSK Cancer Center Support Grant (P30 CA008748) acknowledged in PubMed and PDF -- MSK corresponding author is Jayanta Chaudhuri -- Source: Scopus |
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71Chaudhuri -
17Yewdell -
11Cols Vidal -
8Smolkin
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