G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration Journal Article
| Authors: | Boraas, L. C.; Hu, M.; Martino, P.; Thornton, L.; Vejnar, C. E.; Zhen, G.; Zeng, L.; Parker, D. M.; Cox, A. L.; Giraldez, A. J.; Su, X.; Mayr, C.; Wang, S.; Nicoli, S. |
| Article Title: | G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration |
| Abstract: | The subcellular localization of mRNAs plays a pivotal role in biological processes, including cell migration. For instance, β-actin mRNA and its associated RNA-binding protein (RBP), ZBP1/IGF2BP1, are recruited to focal adhesions (FAs) to support localized β-actin synthesis, crucial for cell migration. However, whether other mRNAs and RBPs also localize at FAs remains unclear. Here, we identify hundreds of mRNAs that are enriched at FAs (FA-mRNAs). FA-mRNAs share characteristics with stress granule (SG) mRNAs and are found in ribonucleoprotein (RNP) complexes with the SG RBP. Mechanistically, G3BP1 binds to FA proteins in an RNA-dependent manner, and its RNA-binding and dimerization domains, essential for G3BP1 to form RNPs in SG, are required for FA localization and cell migration. We find that G3BP1 RNPs promote cell speed by enhancing FA protein mobility and FA size. These findings suggest a previously unappreciated role for G3BP1 RNPs in regulating FA function under non-stress conditions. © 2025 The Authors |
| Keywords: | immunohistochemistry; vasculotropin; protein expression; unclassified drug; human cell; microscopy; protein function; mass spectrometry; confocal microscopy; protein; immunofluorescence; fluorescence in situ hybridization; cell culture; messenger rna; chromatin immunoprecipitation; western blotting; immunoprecipitation; cell migration; fibroblast; real time polymerase chain reaction; ribosome rna; transfer rna; bioinformatics; site directed mutagenesis; rna sequence; ribonucleoprotein; focal adhesions; high throughput sequencing; human; male; article; live cell imaging; g3bp1; cell migration assay; cp: molecular biology; cp: cell biology; huvec cell line; stress granule; protein mobility; ribonucleoprotein complexes; rna localization; ras gtpase activating protein binding protein 1; bosc-23 cell line; phase microscopy |
| Journal Title: | Cell Reports |
| Volume: | 44 |
| Issue: | 2 |
| ISSN: | 2211-1247 |
| Publisher: | Cell Press |
| Date Published: | 2025-02-25 |
| Start Page: | 115237 |
| Language: | English |
| DOI: | 10.1016/j.celrep.2025.115237 |
| PROVIDER: | scopus |
| PUBMED: | 39883578 |
| PMCID: | PMC11923778 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
