DNA aptamers that modulate biological activity of model neurons Journal Article
| Authors: | Rolli, J.; Pearson, K.; Wilbanks, B.; Hrstka, S. C. L.; Minotti, A. P.; Studer, L.; Warrington, A. E.; Staff, N. P.; Maher, L. J. 3rd |
| Article Title: | DNA aptamers that modulate biological activity of model neurons |
| Abstract: | There is an urgent need for agents that promote health and regeneration of cells and tissues, specifically to treat diseases of the aging nervous system. Age-associated nervous system degeneration and various diseases are driven by many different biochemical stresses, often making it difficult to target any one disease cause. Our laboratory has previously identified DNA aptamers with apparent regenerative properties in murine models of multiple sclerosis by selecting aptamers that bind oligodendrocyte membrane preparations. Here, we selected from vast libraries of molecules (∼1014 unique DNAs) those with the ability to bind cultured human SH-SY5Y neuroblastoma cells as a neuronal model, followed by screening for aptamers capable of eliciting biological responses, with validation of binding in differentiated SH-SY5Y, human induced pluripotent stem cell (iPSC)-derived sensory neurons, and human embryonic stem cell (hESC)-derived cortical neurons. This demonstrates a proof-of-concept workflow to identify biologically active aptamers by cycles of cell selection. © 2024 The Author(s) |
| Keywords: | controlled study; human cell; neuromodulation; cell differentiation; in vitro study; molecular library; dna; biological activity; multiple sclerosis; nerve regeneration; cell selection; sensory nerve cell; differentiation; aptamer; in vitro selection; nerve degeneration; oligodendroglia; rna-seq; neurite; induced pluripotent stem cell; human; article; rna sequencing; sh-sy5y; human embryonic stem cell; sh-sy5y cell line; mt: oligonucleotides: therapies and applications |
| Journal Title: | Molecular Therapy - Nucleic Acids |
| Volume: | 35 |
| Issue: | 4 |
| ISSN: | 2162-2531 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2024-12-10 |
| Start Page: | 102392 |
| Language: | English |
| DOI: | 10.1016/j.omtn.2024.102392 |
| PROVIDER: | scopus |
| PMCID: | PMC11667033 |
| PUBMED: | 39720700 |
| DOI/URL: | |
| Notes: | Article -- MSK Cancer Center Support Grant (P30 CA008748) acknowledged in PDF -- Source: Scopus |
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