Authors: | Chow, A.; Lareau, C. A. |
Review Title: | Concepts and new developments in droplet-based single cell multi-omics |
Abstract: | Single cell sequencing technologies have become a fixture in the molecular profiling of cells due to their ease, flexibility, and commercial availability. In particular, partitioning individual cells inside oil droplets via microfluidic reactions enables transcriptomic or multi-omic measurements for thousands of cells in parallel. Complementing the multitude of biological discoveries from genomics analyses, the past decade has brought new capabilities from assay baselines to enable a deeper understanding of the complex data from single cell multi-omics. Here, we highlight four innovations that have improved the reliability and understanding of droplet microfluidic assays. We emphasize new developments that further orient principles of technology development and guidelines for the design, benchmarking, and implementation of new droplet-based methodologies. © 2024 Elsevier Ltd |
Keywords: | human cell; review; nonhuman; cytology; drug development; transcriptomics; microfluidics; genomics; benchmarking; reliability; bioinformatics; microfluidic analytical techniques; molecular profiling; sequencing; cells; single cell analysis; single-cell analysis; procedures; single cells; single cell; microfluidic analysis; individual cells; humans; human; multi-omics; molecular fingerprinting; multiomics; drops; 'omics'; multi-omic; commercial availability; microfluidic reactions; oil droplets |
Journal Title: | Trends in Biotechnology |
Volume: | 42 |
Issue: | 11 |
ISSN: | 0167-7799 |
Publisher: | Elsevier Inc. |
Date Published: | 2024-11-01 |
Start Page: | 1379 |
End Page: | 1395 |
Language: | English |
DOI: | 10.1016/j.tibtech.2024.07.006 |
PUBMED: | 39095258 |
PROVIDER: | scopus |
PMCID: | PMC11568944 |
DOI/URL: | |
Notes: | The MSK Cancer Center Support Grant (P30 CA008748) is acknowledge in the PDF -- Corresponding authors is MSK author: Caleb A. Lareau -- Source: Scopus |