Concepts and new developments in droplet-based single cell multi-omics Review

   


Authors: Chow, A.; Lareau, C. A.
Review Title: Concepts and new developments in droplet-based single cell multi-omics
Abstract: Single cell sequencing technologies have become a fixture in the molecular profiling of cells due to their ease, flexibility, and commercial availability. In particular, partitioning individual cells inside oil droplets via microfluidic reactions enables transcriptomic or multi-omic measurements for thousands of cells in parallel. Complementing the multitude of biological discoveries from genomics analyses, the past decade has brought new capabilities from assay baselines to enable a deeper understanding of the complex data from single cell multi-omics. Here, we highlight four innovations that have improved the reliability and understanding of droplet microfluidic assays. We emphasize new developments that further orient principles of technology development and guidelines for the design, benchmarking, and implementation of new droplet-based methodologies. © 2024 Elsevier Ltd
Keywords: human cell; review; nonhuman; cytology; drug development; transcriptomics; microfluidics; genomics; benchmarking; reliability; bioinformatics; microfluidic analytical techniques; molecular profiling; sequencing; cells; single cell analysis; single-cell analysis; procedures; single cells; single cell; microfluidic analysis; individual cells; humans; human; multi-omics; molecular fingerprinting; multiomics; drops; 'omics'; multi-omic; commercial availability; microfluidic reactions; oil droplets
Journal Title: Trends in Biotechnology
Volume: 42
Issue: 11
ISSN: 0167-7799
Publisher: Elsevier Inc.  
Date Published: 2024-11-01
Start Page: 1379
End Page: 1395
Language: English
DOI: 10.1016/j.tibtech.2024.07.006
PUBMED: 39095258
PROVIDER: scopus
PMCID: PMC11568944
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85200383774&doi=10.1016%2fj.tibtech.2024.07.006&partnerID=40&md5=32fe514eb66860d06ff19a36f0c9d156
Notes: The MSK Cancer Center Support Grant (P30 CA008748) is acknowledge in the PDF -- Corresponding authors is MSK author: Caleb A. Lareau -- Source: Scopus
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MSK Authors
  1. Arthur W Chow
    17 Chow
  2. Caleb A Lareau
    11 Lareau
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