Protocol for generating human cortical organoidsenriched in outer radial glia by guided differentiation Journal Article
| Authors: | Luongo, R.; Walsh, R. M.; Verrillo, A.; Studer, L.; Baggiolini, A. |
| Article Title: | Protocol for generating human cortical organoidsenriched in outer radial glia by guided differentiation |
| Abstract: | The generation of human pluripotent stem cell (hPSC)-derived brain organoids iscontinuously refined, enhancing their reproducibility and complexity. Here, wepresent a guided differentiation protocolfor generating cortical forebrain orga-noids and cortico-pericyte (CP)assembloids composed of a robust outer radialglia (oRG) population and an expanded outer subventricular zone (oSVZ). Wedescribe the steps to generate hPSC-derived cortical organoids (COs), corticalpericytes, and CP assembloids. Moreover, we outline the procedures to charac-terize the organoids by immunostaining and to perform single-cell dissociation.For complete details on the use and execution of this protocol, please refer toWalsh et al. |
| Keywords: | cells |
| Journal Title: | STAR Protocols |
| Volume: | 5 |
| Issue: | 3 |
| ISSN: | 2666-1667 |
| Publisher: | Cell Press |
| Date Published: | 2024-09-20 |
| Start Page: | 103280 |
| Language: | English |
| ACCESSION: | WOS:001315495200001 |
| DOI: | 10.1016/j.xpro.2024.103280 |
| PROVIDER: | wos |
| PMCID: | PMC11401355 |
| PUBMED: | 39213150 |
| Notes: | The MSK Cancer Center Support Grant (P30 CA008748) is acknowledged in the PubMed record -- Source: Wos |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work