Immunohistochemistry staining Book Section
| Authors: | Beaulieu, D.; Giordano, C.; Srivastava, D.; Nehal, K. S.; Nijhawan, R. |
| Editors: | McKay, C.; Nehal, K. S. |
| Article/Chapter Title: | Immunohistochemistry staining |
| Abstract: | Immunohistochemistry (IHC) techniques can be performed in conjunction with Hematoxylin Hematoxylin and Eosin Eosin staining Staining (H&E) and is particularly useful in certain circumstances when tumor identification can be challenging. IHC refers to the process of using an antibody Antibody conjugated with an enzyme probe to target a specific cellular antigen Antigen . Upon binding of the antigen Antigen , the antibody Antibody -enzyme probe complex may either produce a visible reaction product or there may be emission of light or fluorescence, allowing the positively staining Staining cell to be readily identified on light microscopy. The high fidelity with which the antibody Antibody probe binds its antigen and the specificity of the antigen Antigen to certain cell lineages makes IHC a valuable tool in tissue staining Staining , allowing for better delineation of tumors from normal cells. This chapter will serve as a reference for performing IHC staining on Mohs tissue specimens. Particular attention will be paid to IHC for in situ and early invasive melanoma and squamous cell carcinoma. In addition, examples of extramammary Paget disease, basal cell carcinoma, and Merkel cell carcinoma will demonstrate the broad applicability of cytokeratin stains. © The Author(s), Springer Nature Switzerland AG 2024. All rights reserved. |
| Keywords: | antigen; antibody; protocol; negative control; mart-1; positive control; central mohs debulk; cytokeratin staining; cytoplasmic staining; nuclear staining |
| Book Title: | Laboratory manual for mohs micrographic surgery: Frozen tissue processing |
| ISBN: | 978-3-031-52433-2 |
| Publisher: | Springer |
| Publication Place: | Cham, Switzerland |
| Date Published: | 2024-01-01 |
| Start Page: | 163 |
| End Page: | 183 |
| Language: | English |
| DOI: | 10.1007/978-3-031-52434-9_14 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
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