Oral bacteria relative abundance in faeces increases due to gut microbiota depletion and is linked with patient outcomes Journal Article
| Authors: | Liao, C.; Rolling, T.; Djukovic, A.; Fei, T.; Mishra, V.; Liu, H.; Lindberg, C.; Dai, L.; Zhai, B.; Peled, J. U.; van den Brink, M. R. M.; Hohl, T. M.; Xavier, J. B. |
| Article Title: | Oral bacteria relative abundance in faeces increases due to gut microbiota depletion and is linked with patient outcomes |
| Abstract: | The detection of oral bacteria in faecal samples has been associated with inflammation and intestinal diseases. The increased relative abundance of oral bacteria in faeces has two competing explanations: either oral bacteria invade the gut ecosystem and expand (the ‘expansion’ hypothesis), or oral bacteria transit through the gut and their relative increase marks the depletion of other gut bacteria (the ‘marker’ hypothesis). Here we collected oral and faecal samples from mouse models of gut dysbiosis (antibiotic treatment and DSS-induced colitis) and used 16S ribosomal RNA sequencing to determine the abundance dynamics of oral bacteria. We found that the relative, but not absolute, abundance of oral bacteria increases, reflecting the ‘marker’ hypothesis. Faecal microbiome datasets from diverse patient cohorts, including healthy individuals and patients with allogeneic haematopoietic cell transplantation or inflammatory bowel disease, consistently support the ‘marker’ hypothesis and explain associations between oral bacterial abundance and patient outcomes consistent with depleted gut microbiota. By distinguishing between the two hypotheses, our study guides the interpretation of microbiome compositional data and could potentially identify cases where therapies are needed to rebuild the resident microbiome rather than protect against invading oral bacteria. © The Author(s), under exclusive licence to Springer Nature Limited 2024. |
| Keywords: | controlled study; treatment outcome; survival analysis; antibiotic agent; genetics; mortality; nonhuman; mouse; animal; animals; mice; classification; animal experiment; animal model; cohort analysis; mice, inbred c57bl; c57bl mouse; disease model; antiinfective agent; anti-bacterial agents; graft versus host reaction; colitis; real time polymerase chain reaction; hazard ratio; inflammatory bowel diseases; enterococcus; bloodstream infection; vancomycin; disease models, animal; intestine flora; microbiology; rna 16s; isolation and purification; feces; mouth; bacterium; rank sum test; crohn disease; linear regression analysis; bacteria; neomycin; klebsiella; ampicillin; citrobacter; enterobacter; mouth flora; wilcoxon signed ranks test; escherichia; inflammatory bowel disease; bacterial load; dextran sulfate; rna, ribosomal, 16s; humans; human; female; article; dysbiosis; gastrointestinal microbiome; allogeneic haematopoietic cell transplantation; compositional analysis; inverse correlation |
| Journal Title: | Nature Microbiology |
| Volume: | 9 |
| Issue: | 6 |
| ISSN: | 2058-5276 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2024-06-01 |
| Start Page: | 1555 |
| End Page: | 1565 |
| Language: | English |
| DOI: | 10.1038/s41564-024-01680-3 |
| PUBMED: | 38698178 |
| PROVIDER: | scopus |
| PMCID: | PMC11152985 |
| DOI/URL: | |
| Notes: | The MSK Cancer Center Support Grant (P30 CA008748) is acknowledged in [the PubMed record/PDF/the PubMed record and PDF]. Corresponding MSK authors are Tobias M. Hohl and Joao B. Xavier -- Source: Scopus |
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