Structures, mechanisms and applications of RNA-centric CRISPR–Cas13 Review
| Authors: | Yang, H.; Patel, D. J. |
| Review Title: | Structures, mechanisms and applications of RNA-centric CRISPR–Cas13 |
| Abstract: | Prokaryotes are equipped with a variety of resistance strategies to survive frequent viral attacks or invading mobile genetic elements. Among these, CRISPR–Cas surveillance systems are abundant and have been studied extensively. This Review focuses on CRISPR–Cas type VI Cas13 systems that use single-subunit RNA-guided Cas endonucleases for targeting and subsequent degradation of foreign RNA, thereby providing adaptive immunity. Notably, distinct from single-subunit DNA-cleaving Cas9 and Cas12 systems, Cas13 exhibits target RNA-activated substrate RNase activity. This Review outlines structural, biochemical and cell biological studies toward elucidation of the unique structural and mechanistic principles underlying surveillance effector complex formation, precursor CRISPR RNA (pre-crRNA) processing, self-discrimination and RNA degradation in Cas13 systems as well as insights into suppression by bacteriophage-encoded anti-CRISPR proteins and regulation by endogenous accessory proteins. Owing to its programmable ability for RNA recognition and cleavage, Cas13 provides powerful RNA targeting, editing, detection and imaging platforms with emerging biotechnological and therapeutic applications. (Figure presented.) © Springer Nature America, Inc. 2024. |
| Keywords: | signal transduction; genetics; review; nonhuman; protein domain; metabolism; cell death; complex formation; enzyme activity; rna; chemistry; dna; conserved sequence; alternative rna splicing; virus replication; conformational transition; protein secondary structure; endonuclease; adaptive immunity; enzyme mechanism; rna cleavage; rna binding; rna degradation; ribonuclease; bacterial cell; prokaryote; crispr-associated proteins; bacteriophage; bacteriophages; protein engineering; human; crispr associated protein; clustered regularly interspaced short palindromic repeat; crispr cas system; crispr-cas systems; mobile genetic element; clustered regularly interspaced short palindromic repeats; rna, guide, crispr-cas systems; crispr-cas system guide rna |
| Journal Title: | Nature Chemical Biology |
| Volume: | 20 |
| Issue: | 6 |
| ISSN: | 1552-4450 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2024-06-01 |
| Start Page: | 673 |
| End Page: | 688 |
| Language: | English |
| DOI: | 10.1038/s41589-024-01593-6 |
| PUBMED: | 38702571 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- MSK Cancer Center Support Grant (P30 CA008748) acknowledged in PubMed and PDF -- MSK corresponding author is Dinshaw Patel -- Source: Scopus |
