Applying 3D ED/MicroED workflows toward the next frontiers Review
| Authors: | Aragon, M.; Bowman, S. E. J.; Chen, C. H.; de la Cruz, M. J.; Decato, D. A.; Eng, E. T.; Flatt, K. M.; Gulati, S.; Li, Y.; Lomba, C. J.; Mercado, B.; Miller, J.; Palatinus, L.; Rice, W. J.; Waterman, D.; Zimanyi, C. M. |
| Review Title: | Applying 3D ED/MicroED workflows toward the next frontiers |
| Abstract: | We report on the latest advancements in Microcrystal Electron Diffraction (3D ED/MicroED), as discussed during a symposium at the National Center for CryoEM Access and Training housed at the New York Structural Biology Center. This snapshot describes cutting-edge developments in various facets of the field and identifies potential avenues for continued progress. Key sections discuss instrumentation access, research applications for small molecules and biomacromolecules, data collection hardware and software, data reduction software, and finally reporting and validation. 3D ED/MicroED is still early in its wide adoption by the structural science community with ample opportunities for expansion, growth, and innovation. © 2024 International Union of Crystallography. All rights reserved. |
| Keywords: | new york; software; small molecules; cryoelectron microscopy; workflow; structural biology; application programs; biomacromolecule; electron diffraction; microed; microcrystals; work-flows; 3d ed; microcrystal electron diffraction; cutting edges; research applications |
| Journal Title: | Acta Crystallographica Section C-Structural Chemistry |
| Volume: | 80 |
| Issue: | 6 |
| ISSN: | 2053-2296 |
| Publisher: | Int Union Crystallography |
| Date Published: | 2024-06-01 |
| Start Page: | 179 |
| End Page: | 189 |
| Language: | English |
| DOI: | 10.1107/s2053229624004078 |
| PUBMED: | 38712546 |
| PROVIDER: | scopus |
| PMCID: | PMC11150879 |
| DOI/URL: | |
| Notes: | The MSK Cancer Center Support Grant (P30 CA008748) is acknowledged in the PDF -- Source: Scopus |
