Scaling up cryo-EM for biology and chemistry: The journey from niche technology to mainstream method Review
| Authors: | de la Cruz, M. J.; Eng, E. T. |
| Review Title: | Scaling up cryo-EM for biology and chemistry: The journey from niche technology to mainstream method |
| Abstract: | Cryoelectron microscopy (cryo-EM) methods have made meaningful contributions in a wide variety of scientific research fields. In structural biology, cryo-EM routinely elucidates molecular structure from isolated biological macromolecular complexes or in a cellular context by harnessing the high-resolution power of the electron in order to image samples in a frozen, hydrated environment. For structural chemistry, the cryo-EM method popularly known as microcrystal electron diffraction (MicroED) has facilitated atomic structure generation of peptides and small molecules from their three-dimensional crystal forms. As cryo-EM has grown from an emerging technology, it has undergone modernization to enable multimodal transmission electron microscopy (TEM) techniques becoming more routine, reproducible, and accessible to accelerate research across multiple disciplines. We review recent advances in modern cryo-EM and assess how they are contributing to the future of the field with an eye to the past. © 2023 The Authors |
| Keywords: | review; biology; algorithms; chemistry; technology; macromolecule; macromolecular substances; cryoelectron microscopy; microscopy, electron, transmission; transmission electron microscopy; scale up; procedures; cryo-em; machine learning; crystal; electron microscope; electron diffraction; microed; commercialization; cryo-et; data deposition; spa; vitrification; absorption lag time |
| Journal Title: | Structure |
| Volume: | 31 |
| Issue: | 12 |
| ISSN: | 0969-2126 |
| Publisher: | Cell Press |
| Date Published: | 2023-12-01 |
| Start Page: | 1487 |
| End Page: | 1498 |
| Language: | English |
| DOI: | 10.1016/j.str.2023.09.009 |
| PUBMED: | 37820731 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | The MSK Cancer Center Support Grant (P30 CA008748) is acknowledged in the PubMed record and PDF. Corresponding MSK author is M. Jason de la Cruz -- Source: Scopus |
