Pre-processing for precise super-resolution multimodal image analysis Conference Paper
| Authors: | Jang, H.; Li, Z.; Bahrenburg, F.; Ackerstaff, E.; Koutcher, J. A.; Shi, L. |
| Title: | Pre-processing for precise super-resolution multimodal image analysis |
| Conference Title: | SPIE BIOS: Advanced Chemical Microscopy for Life Science and Translational Medicine 2024 |
| Abstract: | We have developed a novel methodology to capture images of various biomolecules at a resolution surpassing the traditional diffraction limit of optical microscopy. By harnessing a multimodal imaging platform that combines stimulated Raman scattering (SRS), multiphoton fluorescence (MPF), and second harmonic generation (SHG), together with sophisticated image deconvolution algorithms, we have successfully generated super-resolution images that reveal the details of biomolecular metabolism. These images enable us to explore the intricate associations between metabolic activities and the spatial distribution of metabolites within breast cancer tissues. To enhance the accuracy of this measurement technique, in this study, we designed a pre-processing workflow that incorporates both denoising and drift correction processes. Our cutting-edge, nonlinear multimodal imaging approach, when applied in a super-resolution context with new workflow, holds significant promise for advancing early detection of breast cancer, prognostication, evaluation of therapeutic outcomes, and deepening our mechanistic understanding of diseases. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only. |
| Keywords: | metabolism; medical imaging; molecular biology; diseases; multi-modal; metabolic imaging; multimodal imaging; metabolites; microscopic examination; optical resolving power; diffraction; multiphotons; deconvolution; cancer; multiphoton; second harmonic generation; nonlinear optics; harmonic generation; pre-processing; multimodal; spatial correlations; superresolution; super resolution; work-flows; stimulated raman scattering; spatial correlation; stimulated raman |
| Journal Title | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume: | 12855 |
| Conference Dates: | 2024 Jan 27-29 |
| Conference Location: | San Francisco, CA |
| ISBN: | 1605-7422 |
| Publisher: | SPIE |
| Date Published: | 2024-01-01 |
| Start Page: | 1285505 |
| Language: | English |
| DOI: | 10.1117/12.3006012 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Conference paper -- Source: Scopus |
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