Mapping the fate of hypoxic cells using an irreversible fluorescent switch Journal Article
| Authors: | Weinstein, A. G.; Gilkes, D. M.; Godet, I.; Gilkes, D. M. |
| Article Title: | Mapping the fate of hypoxic cells using an irreversible fluorescent switch |
| Abstract: | Hypoxia has been reported to promote tumor progression and metastasis in murine models, and patients with hypoxic tumors have a worse prognosis. Besides its effect on cancer, normal processes like embryogenesis, or other pathologies such as ischemia, depend on hypoxia-regulated mechanisms. Given the degradable nature of HIF-1/2α in the presence of oxygen, defining the role of hypoxia in modeling biological processes becomes challenging when a cell enters oxygen-rich regions within a tissue. Here, we describe a unique approach to permanently mark cells that experience hypoxia with a fluorescent protein switch that is maintained even after a cell is reoxygenated. This method consists of a dual-viral delivery system that can be transduced into any mammalian cell line. © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. |
| Keywords: | mouse; animal; animals; mice; metastasis; oxygen; cell line; embryo development; hypoxia; mammal; mammals; embryonic development; cre-loxp; fluorescent reporter; coloring agents; coloring agent; humans; human; hypoxia fate-mapping; hypoxia reporter; intratumoral hypoxia |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 2755 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2024-01-01 |
| Start Page: | 49 |
| End Page: | 61 |
| Language: | English |
| DOI: | 10.1007/978-1-0716-3633-6_3 |
| PUBMED: | 38319568 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | This chapter (3) was published in a book titled "Hypoxia: Methods and Protocols" (ISBN: 978-1-0716-3632-9). It is a volume in the Methods in Molecular Biology series -- Source: Scopus |
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