Successful rearing of nutritionally supplemented rotifers (Brachionus plicatilis) at reduced salinity for zebrafish (Danio rerio) polyculture Journal Article


Authors: Ma, K. G. L.; Lieggi, C.; Lertpiriyapong, K.; Afolalu, A. A.; Riedel, E. R.; Lipman, N. S.
Article Title: Successful rearing of nutritionally supplemented rotifers (Brachionus plicatilis) at reduced salinity for zebrafish (Danio rerio) polyculture
Abstract: Rotifers, Brachionus plicatilis, are a valuable first exogenous feed for zebrafish because they can provide continuous nutrition for growing zebrafish larvae when used in a rotifer-zebrafish polyculture. Typically cultured at high salinities (>10 ppt), B. plicatilis are temporarily immobilized when moved to lower salinities (5 ppt) used for polycultures, decreasing their accessibility and attractiveness to the larvae. The nutritional value of rotifers varies based on their diet, typically live algae, which has limited nutritional value and may pose biosecurity risks. After confirming that rotifers consume and can reproduce when fed an irradiated, processed larval fish diet (PD), they were reared at 5 or 15 ppt, and fed various combinations of an algae mix and/or PD. Population densities and percentages of egg-bearing rotifers were quantified daily until the population density plateaued, and then their nutritional value was assessed. Results indicated that rotifers thrived at both salinities. Those fed PD were successfully maintained at >500 rotifers per mL and contained a greater ω-6/ω-3 fatty acid ratio. Our findings indicate that enriching rotifers with PD raised at 5 ppt can potentially eliminate rotifer immobilization in polyculture, while providing a nutritious, attractive diet for zebrafish larvae and decreasing biosecurity risks.
Keywords: animal; animals; dietary supplements; dietary supplement; zebra fish; zebrafish; larva; husbandry; brachionus; polyculture; rotifers; perciformes; rotifera; salinity
Journal Title: Zebrafish
Volume: 20
Issue: 6
ISSN: 1545-8547
Publisher: Mary Ann Liebert, Inc  
Date Published: 2023-12-01
Start Page: 250
End Page: 259
Language: English
DOI: 10.1089/zeb.2023.0027
PUBMED: 38117218
PROVIDER: scopus
PMCID: PMC10733754
DOI/URL:
Notes: The MSK Cancer Center Support Grant (P30 CA008748) is acknowledged in the PDF -- Source: Scopus
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MSK Authors
  1. Neil S Lipman
    86 Lipman
  2. Christine Lieggi
    17 Lieggi
  3. Kathleen Git-ling Ma
    2 Ma