Targeting intracellular antigens with pMHC-binding antibodies: A phage display approach Journal Article
| Authors: | Yang, Z.; Wu, Z.; Santich, B. H.; Liu, J.; Liu, C.; Cheung, N. K. V. |
| Article Title: | Targeting intracellular antigens with pMHC-binding antibodies: A phage display approach |
| Abstract: | Antibodies that bind peptide-MHC (pMHC) complex in a manner akin to T cell receptor (TCR) have not only helped in understanding the mechanism of TCR-pMHC interactions in the context of T cell biology but also spurred considerable interest in recent years as potential cancer therapeutics. Traditional methods to generate such antibodies using hybridoma and B cell sorting technologies are sometimes inadequate, possibly due to the small contribution of peptide to the overall B cell epitope space on the surface of the pMHC complex (typical peptide MW = 1 kDa versus MHC MW = 45 kDa) and to the multiple efficiency limiting steps inherent in these methods. In this chapter we describe phage display approaches, including a cell panning strategy, for the rapid generation of such antibodies with high specificity and affinity. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. |
| Keywords: | protein expression; cell motion; b lymphocyte; b-lymphocytes; cell movement; antibodies; major histocompatibility complex; antibody; phage display; histocompatibility antigens; histocompatibility antigen; bacteriophage; bacteriophages; human leukocyte antigen; scfv; phage; single-chain variable fragment; t-cell receptor; fc-fusion protein; peptide mhc; cell surface display techniques; cell surface display |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 2702 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2023-01-01 |
| Start Page: | 327 |
| End Page: | 345 |
| Language: | English |
| DOI: | 10.1007/978-1-0716-3381-6_17 |
| PUBMED: | 37679628 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Chapter 17 in "Phage Display: Methods and Protocols. 2nd ed" (ISBN: 978-1-0716-3380-9) -- Source: Scopus |
