Targeting intracellular antigens with pMHC-Binding antibodies: A phage display approach Journal Article
| Authors: | Wu, Z.; Santich, B. H.; Liu, H.; Liu, C.; Cheung, N. K. V. |
| Article Title: | Targeting intracellular antigens with pMHC-Binding antibodies: A phage display approach |
| Abstract: | Antibodies that bind peptide-MHC (pMHC) complex in a manner akin to T-cell receptor (TCR) have not only helped in understanding the mechanism of TCR-pMHC interactions in the context of T-cell biology, but also spurred considerable interest in recent years as potential cancer therapeutics. Traditional methods to generate such antibodies using hybridoma and B-cell sorting technologies are sometimes inadequate, possibly due to the small contribution of peptide to the overall B-cell epitope space on the surface of the pMHC complex (typical peptide MW = 1 kDa versus MHC MW = 45 kDa), and to the multiple efficiency limiting steps inherent in these methods. In this chapter, we describe a phage display approach for the rapid generation of such antibodies with high specificity and affinity. © Springer Science+Business Media LLC 2018. |
| Keywords: | protein expression; protein targeting; protein binding; ph; molecular cloning; t lymphocyte receptor; antigen; hybrid protein; epitope; antibody specificity; antigen binding; major histocompatibility complex; transient transfection; antibody; phage display; antibody affinity; plasmid dna; surface property; human leukocyte antigen; scfv; phage; single-chain variable fragment; t-cell receptor; fc-fusion protein; peptide mhc |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 1701 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2018-01-01 |
| Start Page: | 255 |
| End Page: | 269 |
| Language: | English |
| DOI: | 10.1007/978-1-4939-7447-4_13 |
| PROVIDER: | scopus |
| PUBMED: | 29116509 |
| DOI/URL: | |
| Notes: | Book Chapter 13 in "Phage Display: Methods and Protocols" (ISBN: 978-1-4939-7446-7) -- Export Date: 4 December 2017 -- Source: Scopus |
