19-color, 21-antigen single tube for efficient evaluation of B- and T-cell neoplasms Journal Article
| Authors: | Chan, A.; Gao, Q.; Roshal, M. |
| Article Title: | 19-color, 21-antigen single tube for efficient evaluation of B- and T-cell neoplasms |
| Abstract: | Non-Hodgkin lymphoma (NHL) is a heterogeneous disease, encompassing a wide variety of individually distinct neoplastic entities of mature B-, T-, and NK-cells. While they constitute a broad category, they are the most common hematologic malignancies in the world. The distinction between different neoplastic entities requires a multi-modal approach, such as flow cytometric immunophenotyping, which can exclude a neoplastic proliferation and help narrow the differential diagnosis. This article describes a flow cytometric test developed at Memorial Sloan Kettering Cancer Center to assess B-, T-, and NK-cells in a single tube, 21-antibody, 19-color assay. The assay can identify most B- and T-cell NHLs with high specificity and sensitivity and significantly narrow the differential when a specific diagnosis cannot be made. The basic protocol provides a detailed operational procedure for sample processing, staining, and cytometric acquisition. The support protocol provides typical steps and caveats for data analysis in lymphoproliferative disorders and in discriminating a variety of specific disease entities from each other and normal lymphoid populations. © 2023 Wiley Periodicals LLC. Basic Protocol: Processing, staining, and cytometric analysis of samples for B- and T-cell assessment. Support Protocol: Analysis and interpretation of the B- and T-cell lymphocyte assay. © 2023 Wiley Periodicals LLC. |
| Keywords: | hematologic neoplasms; lymphoma, non-hodgkin; lymphoma; antibodies; antibody; hematologic disease; non-hodgkin lymphoma; t-cells; b-cells; lymphoproliferative disorder; humans; human; lymphoid leukemia; excipient; excipients |
| Journal Title: | Current Protocols |
| Volume: | 3 |
| Issue: | 9 |
| ISSN: | 2691-1299 |
| Publisher: | John Wiley & Sons, Inc. |
| Date Published: | 2023-09-01 |
| Start Page: | e884 |
| Language: | English |
| DOI: | 10.1002/cpz1.884 |
| PUBMED: | 37725693 |
| PROVIDER: | scopus |
| PMCID: | PMC10516508 |
| DOI/URL: | |
| Notes: | Article -- MSK Cancer Center Support Grant (P30 CA008748) acknowledged in PubMed and PDF -- MSK corresponding author is Mikhail Roshal -- Source: Scopus |
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