Standardizing HER2 immunohistochemistry assessment: Calibration of color and intensity variation in whole slide imaging caused by staining and scanning Journal Article
| Authors: | Ohnishi, C.; Ohnishi, T.; Ntiamoah, P.; Ross, D. S.; Yamaguchi, M.; Yagi, Y. |
| Article Title: | Standardizing HER2 immunohistochemistry assessment: Calibration of color and intensity variation in whole slide imaging caused by staining and scanning |
| Abstract: | In the evaluation of human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC) — one of the standard biomarkers for breast cancer— visual assessment is laborious and subjective. Image analysis using whole slide image (WSI) could produce more consistent results; however, color variability in WSIs due to the choice of stain and scanning processes may impact image analysis. We therefore developed a calibration protocol to diminish the staining and scanning variations of WSI using two calibrator slides. The IHC calibrator slide (IHC-CS) contains peptide-coated microbeads with different concentrations. The color distribution obtained from the WSI of stained IHC-CS reflects the staining process and scanner characteristics. A color chart slide (CCS) is also useful for calibrating the color variation due to the scanner. The results of the automated HER2 assessment were compared to confirm the effectiveness of two calibration slides. The IHC-CS and HER2 breast cancer cases were stained on different days. All stained slides and CCS were digitized by two different WSI scanners. Results revealed 100% concordance between automated evaluation and the pathologist’s assessment with both the scanner and staining calibration. The proposed method may enable consistent evaluation of HER2. © 2023, Korean Society of Microscopy. |
| Keywords: | immunohistochemistry; breast cancer; image analysis; epidermal growth factor receptor 2; calibration; medical imaging; diseases; color; human epidermal growth factor; image-analysis; immunohistochemistry (ihc); whole slide images; color and stain intensity calibration; whole slide image (wsi); color variations; whole slide image |
| Journal Title: | Applied Microscopy |
| Volume: | 53 |
| Issue: | 8 |
| ISSN: | 2287-4445 |
| Publisher: | Springer Nature |
| Date Published: | 2023-12-01 |
| Language: | English |
| DOI: | 10.1186/s42649-023-00091-8 |
| PROVIDER: | scopus |
| PMCID: | PMC10499734 |
| PUBMED: | 37704877 |
| DOI/URL: | |
| Notes: | The MSK Cancer Center Support Grant (P30 CA008748) is acknowledged in the PDF -- Corresponding author is MSK author: Chie Ohnishi -- Source: Scopus |
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