Enhancing intracellular optical performance and stability of engineered nanomaterials via aqueous two-phase purification Journal Article
| Authors: | Nadeem, A.; Kindopp, A.; Wyllie, I.; Hubert, L.; Joubert, J.; Lucente, S.; Randall, E.; Jena, P. V.; Roxbury, D. |
| Article Title: | Enhancing intracellular optical performance and stability of engineered nanomaterials via aqueous two-phase purification |
| Abstract: | Supramolecular hybrids of DNA and single-walled carbon nanotubes (SWCNTs) have been introduced in numerous biosensing applications due to their unique optical properties. Recent aqueous two-phase (ATP) purification methods for SWCNTs have gained popularity by introducing specificity and homogeneity into the sensor design process. Using murine macrophages probed by near-infrared and Raman microscopies, we show that ATP purification increases the retention time of DNA-SWCNTs within cells while simultaneously enhancing the optical performance and stability of the engineered nanomaterial. Over a period of 6 h, we observe 45% brighter fluorescence intensity and no significant change in emission wavelength of ATP-purified DNA-SWCNTs relative to as-dispersed SWCNTs. These findings provide strong evidence of how cells differentially process engineered nanomaterials depending on their state of purification, lending to the future development of more robust and sensitive biosensors with desirable in vivo optical parameters using surfactant-based ATP systems with a subsequent exchange to biocompatible functionalization. © 2023 American Chemical Society |
| Keywords: | mouse; animal; animals; mice; fluorescence; dna; biocompatibility; nanoparticles; water; adenosine triphosphate; optical properties; infrared devices; surfactant; carbon nanotube; nanotubes, carbon; nanomaterial; nanostructures; purification; single-walled carbon nanotubes (swcn); near-infrared fluorescence; surface-active agents; aqueous two-phase purification; biosensing; nanoparticle exocytosis; nanoparticle optical stability; aqueous two phase; engineered nanomaterials; optical performance; optical stability; single-walled carbon |
| Journal Title: | Nano Letters |
| Volume: | 23 |
| Issue: | 14 |
| ISSN: | 1530-6984 |
| Publisher: | American Chemical Society |
| Date Published: | 2023-07-26 |
| Start Page: | 6588 |
| End Page: | 6595 |
| Language: | English |
| DOI: | 10.1021/acs.nanolett.3c01727 |
| PUBMED: | 37410951 |
| PROVIDER: | scopus |
| PMCID: | PMC11068083 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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