Integration of whole transcriptome spatial profiling with protein markers Journal Article

   


Authors: Ben-Chetrit, N.; Niu, X.; Swett, A. D.; Sotelo, J.; Jiao, M. S.; Stewart, C. M.; Potenski, C.; Mielinis, P.; Roelli, P.; Stoeckius, M.; Landau, D. A.
Article Title: Integration of whole transcriptome spatial profiling with protein markers
Abstract: Spatial transcriptomics and proteomics provide complementary information that independently transformed our understanding of complex biological processes. However, experimental integration of these modalities is limited. To overcome this, we developed Spatial PrOtein and Transcriptome Sequencing (SPOTS) for high-throughput simultaneous spatial transcriptomics and protein profiling. Compared with unimodal measurements, SPOTS substantially improves signal resolution and cell clustering and enhances the discovery power in differential gene expression analysis across tissue regions. © 2023, The Author(s), under exclusive licence to Springer Nature America, Inc.
Keywords: high-throughput; proteins; gene expression; proteomics; transcriptomics; molecular biology; biological process; transcriptomes; protein markers; cell clustering; protein profiling; signal resolution; unimodal
Journal Title: Nature Biotechnology
Volume: 41
Issue: 6
ISSN: 1087-0156
Publisher: Nature Publishing Group  
Date Published: 2023-06-01
Start Page: 788
End Page: 793
Language: English
DOI: 10.1038/s41587-022-01536-3
PUBMED: 36593397
PROVIDER: scopus
PMCID: PMC10272089
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145487993&doi=10.1038%2fs41587-022-01536-3&partnerID=40&md5=8655b698b73eb88e6f2fa7517452f0c0
Notes: Article -- Source: Scopus
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  1. Maria Socorro Frankowski
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