Spatially resolved transcriptomics reveals the architecture of the tumor-microenvironment interface Journal Article

   

Authors:	Hunter, M. V.; Moncada, R.; Weiss, J. M.; Yanai, I.; White, R. M.
Article Title:	Spatially resolved transcriptomics reveals the architecture of the tumor-microenvironment interface
Abstract:	During tumor progression, cancer cells come into contact with various non-tumor cell types, but it is unclear how tumors adapt to these new environments. Here, we integrate spatially resolved transcriptomics, single-cell RNA-seq, and single-nucleus RNA-seq to characterize tumor-microenvironment interactions at the tumor boundary. Using a zebrafish model of melanoma, we identify a distinct “interface” cell state where the tumor contacts neighboring tissues. This interface is composed of specialized tumor and microenvironment cells that upregulate a common set of cilia genes, and cilia proteins are enriched only where the tumor contacts the microenvironment. Cilia gene expression is regulated by ETS-family transcription factors, which normally act to suppress cilia genes outside of the interface. A cilia-enriched interface is conserved in human patient samples, suggesting it is a conserved feature of human melanoma. Our results demonstrate the power of spatially resolved transcriptomics in uncovering mechanisms that allow tumors to adapt to new environments. © 2021, The Author(s).
Keywords:	gene expression; protein; rna; tumor; danio rerio; detection method; cancer
Journal Title:	Nature Communications
Volume:	12
ISSN:	2041-1723
Publisher:	Nature Publishing Group
Date Published:	2021-11-01
Start Page:	6278
Language:	English
DOI:	10.1038/s41467-021-26614-z
PROVIDER:	scopus
PMCID:	PMC8560802
PUBMED:	34725363
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118454855&doi=10.1038%2fs41467-021-26614-z&partnerID=40&md5=307d32bd8f28c8be2e2ca5e4a5271ff6
Notes:	Article -- Export Date: 1 December 2021 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/190461