Assessing in vivo presentation of exogenous antigen in the tumor microenvironment in mice Journal Article

   


Authors: Nixon, B. G.; Ji, L.; Li, M. O.
Article Title: Assessing in vivo presentation of exogenous antigen in the tumor microenvironment in mice
Abstract: Here, we focus on tumor-associated macrophages (TAMs) in the PyMT model of breast cancer, detailing a protocol for assessing antigen presentation capabilities of immune populations of interest. We describe a stringent bone marrow chimera system to demonstrate presentation of exogenous antigen that is acquired and processed in the tumor microenvironment. We describe steps for testing antigen presentation activity of TAMs to CD8+ T cells in vivo and ex vivo and the requirement for the transcription factor IRF8 in this function. For complete details on the use and execution of this protocol, please refer to Nixon et al. (2022).1 © 2023 The Author(s)
Keywords: immunology; cell culture; cell isolation; model organisms; cell biology; cancer; flow cytometry/mass cytometry
Journal Title: STAR Protocols
Volume: 4
Issue: 2
ISSN: 2666-1667
Publisher: Cell Press  
Date Published: 2023-06-16
Start Page: 102185
Language: English
DOI: 10.1016/j.xpro.2023.102185
PROVIDER: scopus
PMCID: PMC10060903
PUBMED: 36952337
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85150348937&doi=10.1016%2fj.xpro.2023.102185&partnerID=40&md5=e1ca42ff07e5edfc9b7eb19e1fb45cfe
Notes: Article -- MSK corresponding authors are Briana Nixon and Liangliang Ji -- Source: Scopus
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MSK Authors
  1. Ming Li
    110 Li
  2. Briana Glyn Nixon
    24 Nixon
  3. Liangliang Ji
    6 Ji
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