Protocol for the isolation of tumor cell-derived extracellular vesicles followed by in vivo metastasis assessment in a murine ovarian cancer model Journal Article
| Authors: | Mittal, S.; Kumar, S.; Gupta, P.; Singh, M.; Chaluvally-Raghavan, P.; Pradeep, S. |
| Article Title: | Protocol for the isolation of tumor cell-derived extracellular vesicles followed by in vivo metastasis assessment in a murine ovarian cancer model |
| Abstract: | Extracellular vesicles (EVs) play a crucial role in facilitating communication between cancer cells and their immediate or remote microenvironments, thereby promoting the extensive spread of cancer throughout the body. In this context, we present a protocol for the isolation of tumor cell-derived EVs followed by in vivo metastasis assessment in a murine ovarian cancer model. We describe steps for the isolation and characterization of EVs from ID8 cells, development of a metastatic mouse model, and sample preparation for flow cytometry. For complete details on the use and execution of this protocol, please refer to Gupta et al.1 © 2024 The Authors |
| Keywords: | controlled study; human cell; cancer growth; nonhuman; flow cytometry; cell proliferation; animal cell; mouse; animal tissue; diagnostic procedure; metastasis; apoptosis; ovary cancer; bleeding; green fluorescent protein; animal experiment; animal model; immunology; genetic transfection; cancer cell; western blotting; lentivirus vector; fluorescence activated cell sorting; programmed death 1 receptor; tumor microenvironment; transmission electron microscopy; exosome; sphingosine kinase 1; cervical spine dislocation; ultracentrifugation; cancer; human; female; article; hek293t cell line; hepatitis a virus cellular receptor 2; id8 cell line; nanoparticle tracking analysis |
| Journal Title: | STAR Protocols |
| Volume: | 5 |
| Issue: | 2 |
| ISSN: | 2666-1667 |
| Publisher: | Cell Press |
| Date Published: | 2024-06-21 |
| Start Page: | 102943 |
| Language: | English |
| DOI: | 10.1016/j.xpro.2024.102943 |
| PUBMED: | 38470912 |
| PROVIDER: | scopus |
| PMCID: | PMC10945248 |
| DOI/URL: | |
| Notes: | Source: Scopus |
