Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter strains in China: A multicenter genomic study Journal Article
| Authors: | Zhu, Z.; Xie, X.; Yu, H.; Jia, W.; Shan, B.; Huang, B.; Qu, F.; Niu, S.; Lv, J.; Gao, Q.; Qian, F.; Tian, X.; Zhai, Y.; Wen, Y.; Yang, C.; Zhu, J.; Tang, Y.; Chen, L.; Du, H. |
| Article Title: | Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter strains in China: A multicenter genomic study |
| Abstract: | Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter (CR-Ent) species remain unclear in China. In this study, we performed a genomic study on 92 isolates from Enterobacter-caused infections from a multicenter study in China. Whole genome sequencing (WGS) was used to determine the genome sequence of 92 non-duplicated CR-Ent strains collected from multiple tertiary health centres. The precise species of Enterobacter strains were identified by average nucleotide identity (ANI) and in silico DNA–DNA hybridization (isDDH). Molecular features of high-risk CR-Ent sequence type (ST) lineages and carbapenemase-encoding plasmids were determined. The result revealed that the most common human-source CR-Ent species in China was E. xiangfangensis (66/92, 71.93%), and the proportion of carbapenemase-producing Enterobacter (CP-Ent) in CR-Ent was high (72/92, 78.26%) in comparison to other global regions. Furthermore, ST171 and ST116 E. xiangfangensis were the major lineages of CP-Ent strains, and ST171 E. xiangfangensis was more likely to cause infections in older patients. Genomic analysis also highlighted the likelihood of intra-hospital/inter-hospital clonal transmission of ST171 and ST116 E. xiangfangensis. In addition, the bla NDM-harbouring IncX3-type plasmid was identified as the prevalent carbapenemase-encoding plasmid carried by CR-Ent strains, and was experimentally confirmed to be able to self-transfer with high frequency. This study detailed the genomic and clinical characteristics of CR-Ent in China in the form of multicenter for the first time. The high risk of carbapenemase-producing ST171 and ST116 E. xiangfangensis, and the bla NDM-harbouring IncX3-type plasmid were detected and emphasized. © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. |
| Keywords: | adult; controlled study; aged; unclassified drug; gene sequence; major clinical study; genetics; clinical feature; clinical trial; comparative study; prevalence; bacterial strain; antiinfective agent; bacterial protein; antibiotic resistance; anti-bacterial agents; bacterial proteins; dna; multicenter study; nucleotide sequence; plasmid; genomics; plasmids; high risk population; hospital infection; molecular biology; bacterial dna; bacterium identification; bacterial transmission; genomic dna; bacterium isolation; china; nucleotide; colistin; quinolone; geographic distribution; aminoglycoside; carbapenem; enterobacter; health center; microbial sensitivity tests; carbapenem derivative; beta-lactamases; growth curve; epidemiological data; enterobacteriaceae infections; very elderly; humans; human; male; female; article; whole genome sequencing; microbial sensitivity test; enterobacteriaceae infection; carbapenem-resistant enterobacteriaceae; carbapenemase; dna dna hybridization; carbapenems; beta lactamase; bla ndm; incx3; st116; st171; acra protein; beta lactamase imp 4; beta lactamase kpc 4; beta lactamase ndm; beta lactamase ndm 1; beta lactamase ndm 5; beta lactamase vim 1; catii protein; cgsd protein; csgg protein; ente protein; enterochelin; fepd protein; flor protein; hcp protein; mcr 9 protein; qepa protein; qnrb protein; tet d protein; tssb protein; tssc protein; tssd protein; tssf protein; ugd protein; vipa protein; vipb protein; enterobacter xiangfangensis |
| Journal Title: | Emerging Microbes & Infections |
| Volume: | 12 |
| ISSN: | 2222-1751 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2023-01-01 |
| Start Page: | e2148562 |
| Language: | English |
| DOI: | 10.1080/22221751.2022.2148562 |
| PUBMED: | 36382635 |
| PROVIDER: | scopus |
| PMCID: | PMC9769138 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 February 2023 -- Source: Scopus |
