Evaluation of media conductivity and a combination of iodine and sodium hypochlorite surface disinfection on zebrafish (Danio rerio) embryo viability and morphology Journal Article
| Authors: | Mendoza, G. Jr; Afolalu, A. A.; Lertpiriyapong, K.; Lipman, N. S.; Lieggi, C. |
| Article Title: | Evaluation of media conductivity and a combination of iodine and sodium hypochlorite surface disinfection on zebrafish (Danio rerio) embryo viability and morphology |
| Abstract: | Embryo surface disinfection in either an iodine or sodium hypochlorite (NaOCl) solution is commonly performed on imported zebrafish embryos to decrease pathogen introduction into a facility. The impact of the consecutive use of these disinfectants and the conductivity of the culture media on embryo survival and development post-disinfection have not been evaluated. Iodine (12.5-25 ppm) is effective at eliminating several Mycobacterium species, whereas NaOCl (50-100 ppm) reduces the number of viable Pseudoloma neurophilia spores. Casper and T5D (tropical 5D wild type) embryos reared in media of differing conductivities (0-10, 100-200, 750-950, and 1500-2000 μS) with and without exposure to NaOCl 100 ppm at 6 h post-fertilization were evaluated for survival, hatching success, and morphological defects at 5 days post-fertilization. Additionally, the consecutive use of iodine (12.5 ppm for 2 min) followed by NaOCl (75 or 100 ppm for 10 min), as well as the inverse, was evaluated. Embryo survival was not impacted by embryo rearing media alone; however, survival significantly decreased when embryos were disinfected with 100 ppm NaOCl in media with a conductivity >750-950 μS. Iodine (12 ppm) and NaOCl (75 ppm) used sequentially resulted in >50% survival, whereas the use of 100 ppm NaOCl resulted in high levels of embryo mortality. © 2022 Mary Ann Liebert, Inc., publishers. |
| Keywords: | animal; animals; culture medium; zebra fish; zebrafish; culture media; iodine; disinfection; conductivity; procedures; disinfectant agent; disinfectants; hypochlorite sodium; embryo disinfection; sodium hypochlorite |
| Journal Title: | Zebrafish |
| Volume: | 19 |
| Issue: | 5 |
| ISSN: | 1545-8547 |
| Publisher: | Mary Ann Liebert, Inc |
| Date Published: | 2022-10-01 |
| Start Page: | 190 |
| End Page: | 199 |
| Language: | English |
| DOI: | 10.1089/zeb.2022.0028 |
| PUBMED: | 36206234 |
| PROVIDER: | scopus |
| PMCID: | PMC9595623 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 December 2022 -- Source: Scopus |
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