Impact of pronase, sodium thiosulfate, and methylene blue combinations on development and survival of sodium hypochlorite surface-disinfected Zebrafish (Danio rerio) embryos Journal Article

   

Authors:	Peneyra, S. M.; Lerpiriyapong, K.; Riedel, E. R.; Lipman, N. S.; Lieggi, C.
Article Title:	Impact of pronase, sodium thiosulfate, and methylene blue combinations on development and survival of sodium hypochlorite surface-disinfected Zebrafish (Danio rerio) embryos
Abstract:	Embryo surface disinfection is utilized in aquaculture to decrease the risk of pathogen introduction into established colonies. Zebrafish embryos are commonly disinfected with unbuffered sodium hypochlorite at 25-50 ppm for 10 min with or without concurrent treatment with chemicals, including pronase (Pron), sodium thiosulfate, and/or methylene blue; however, the impact of these chemicals on embryo survival and development has not been evaluated. In this study, AB and casper embryos were exposed to disinfection protocols that used Pron, sodium thiosulfate, and/or methylene blue (given alone, in various combinations, or all three combined) with 50 and 100 ppm sodium hypochlorite performed 6 and 24 h postfertilization (HPF). All groups were evaluated for survival, hatching, and malformations at 5 days postfertilization. Maximal survival (69%-97%) and hatching rates (66%-94%) were generally observed with sodium hypochlorite disinfection followed by exposure to both Pron and sodium thiosulfate and maintenance in standard embryo medium without methylene blue. Methylene blue had variable effects on survival and hatching. Higher survival and hatching rates were seen in AB embryos disinfected at 6 HPF and casper embryos disinfected at 24 HPF. Susceptibility to sodium hypochlorite toxicity differed by strain, emphasizing the need to test disinfection protocols on small embryo cohorts. © Mary Ann Liebert, Inc.
Keywords:	methylene blue; zebrafish; pronase; sodium thiosulfate; embryo disinfection; sodium hypochlorite
Journal Title:	Zebrafish
Volume:	17
Issue:	5
ISSN:	1545-8547
Publisher:	Mary Ann Liebert, Inc
Date Published:	2020-10-01
Start Page:	342
End Page:	353
Language:	English
DOI:	10.1089/zeb.2020.1917
PUBMED:	33048660
PROVIDER:	scopus
PMCID:	PMC7578187
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092885836&doi=10.1089%2fzeb.2020.1917&partnerID=40&md5=346ff94e3a21bb9768c1200a9053876c
Notes:	Article -- Export Date: 2 November 2020 -- Source: Scopus

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