Biochemical, genomic, and epigenomic profiling of isolated cancer cell lines’ micronuclei Journal Article

  

Authors:	Agustinus, A. S.; Bakhoum, S.
Article Title:	Biochemical, genomic, and epigenomic profiling of isolated cancer cell lines’ micronuclei
Abstract:	Micronuclei are common byproducts of chromosomally unstable cancer cells during their division. Micronuclei play an important role in cancer metastasis as well as introducing chromosomal abnormalities into the primary nucleus in the subsequent cell cycle. Given their major role in tumor initiation and progression, methods to examine their properties are crucially needed. This chapter discusses approaches and methods to profile micronuclei's biochemical, genomic, and epigenomic properties following their physical isolation. Using either MPS1 inhibition or radiation to induce formation of micronuclei, this method introduces a versatile way to investigate biological events that occur in micronuclei as well as compare them to events in the primary nuclei from the same system. © 2022 Elsevier Inc.
Keywords:	genetics; neoplasm; neoplasms; mass spectrometry; cell line; epigenetics; genomics; chromosomal instability; genome; epigenomics; humans; human; whole genome sequencing; atac-seq; histone mass spectrometry; micronuclei isolation; whole-genome bisulfite sequencing
Journal Title:	Methods in Cell Biology
Volume:	172
ISSN:	0091-679X
Publisher:	Academic Press, Elsevier Inc
Date Published:	2022-01-01
Start Page:	51
End Page:	66
Language:	English
DOI:	10.1016/bs.mcb.2022.07.001
PUBMED:	36064226
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85136746825&doi=10.1016%2fbs.mcb.2022.07.001&partnerID=40&md5=55cf9336137eab202e1b1eb1f40c4ec0
Notes:	Book Chapter 4 in "Radiation Oncology and Radiotherapy. Part A" (ISBN: 978-0-323-89949-9) -- Export Date: 3 October 2022 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/198193