Methods to assess helicase and translocation activities of human nuclear RNA exosome and RNA adaptor complexes Journal Article

   


Authors: Puno, M. R.; Lima, C. D.
Article Title: Methods to assess helicase and translocation activities of human nuclear RNA exosome and RNA adaptor complexes
Abstract: The nuclear RNA exosome collaborates with the MTR4 helicase and RNA adaptor complexes to process, surveil, and degrade RNA. Here we outline methods to characterize RNA translocation and strand displacement by exosome-associated helicases and adaptor complexes using fluorescence-based strand displacement assays. The design and preparation of substrates suitable for analysis of helicase and decay activities of reconstituted MTR4–exosome complexes are described. To aid structural and biophysical studies, we present strategies for engineering substrates that can stall helicases during translocation, providing a means to capture snapshots of interactions and molecular steps involved in substrate translocation and delivery to the exosome. © 2022 Elsevier Inc.
Keywords: helicase; translocase; dexh-box; rna exosome; sf2; rna–protein complex
Journal Title: Methods in Enzymology
Volume: 673
ISSN: 0076-6879
Publisher: Academic Press  
Date Published: 2022-01-01
Start Page: 453
End Page: 473
Language: English
DOI: 10.1016/bs.mie.2022.03.060
PUBMED: 35965016
PROVIDER: scopus
PMCID: PMC9382703
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85130069427&doi=10.1016%2fbs.mie.2022.03.060&partnerID=40&md5=b4a6e5c59b1e88500ea8b7eb6d67d728
Notes: Book Chapter 16 in "Helicase Enzymes Part B" (ISBN: 978-0-323-99773-7) -- Source: Scopus
Altmetric
What is Altmetric?
Citation Impact
What is Dimensions Citation Badge?
BMJ Impact Analytics
MSK Authors
  1. Christopher D Lima
    103 Lima
  2. Marc Rhyan Jimenez Puno
    8 Puno
Related MSK Work