Substrate discrimination and quality control require each catalytic activity of TRAMP and the nuclear RNA exosome Journal Article


Authors: Das, M.; Zattas, D.; Zinder, J. C.; Wasmuth, E. V.; Henri, J.; Lima, C. D.
Article Title: Substrate discrimination and quality control require each catalytic activity of TRAMP and the nuclear RNA exosome
Abstract: Quality control requires discrimination between functional and aberrant species to selectively target aberrant substrates for destruction. Nuclear RNA quality control in Saccharomyces cerevisiae includes the TRAMP complex that marks RNA for decay via polyadenylation followed by helicase-dependent 3' to 5' degradation by the RNA exosome. Using reconstitution biochemistry, we show that polyadenylation and helicase activities of TRAMP cooperate with processive and distributive exoribonuclease activities of the nuclear RNA exosome to protect stable RNA from degradation while selectively targeting and degrading less stable RNA. Substrate discrimination is lost when the distributive exoribonuclease activity of Rrp6 is inactivated, leading to degradation of stable and unstable RNA species. These data support a proofreading mechanism in which deadenylation by Rrp6 competes with Mtr4- dependent degradation to protect stable RNA while selectively targeting and degrading unstable RNA. © 2021 National Academy of Sciences. All rights reserved.
Keywords: quality control; rna; exosome; rna decay; tramp
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 118
Issue: 14
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 2021-04-06
Start Page: e2024846118
Language: English
DOI: 10.1073/pnas.2024846118
PUBMED: 33782132
PROVIDER: scopus
PMCID: PMC8040639
DOI/URL:
Notes: Article -- Export Date: 3 May 2021 -- Source: Scopus
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MSK Authors
  1. Christopher D Lima
    91 Lima
  2. Elizabeth Wasmuth
    10 Wasmuth
  3. John C Zinder
    6 Zinder
  4. Dimitrios Zattas
    3 Zattas
  5. Mom Das
    3 Das
  6. Julien Benjamin Henri
    1 Henri