Human liver organoids for disease modeling of fibrolamellar carcinoma Journal Article

   


Authors: Narayan, N. J. C.; Requena, D.; Lalazar, G.; Ramos-Espiritu, L.; Ng, D.; Levin, S.; Shebl, B.; Wang, R.; Hammond, W. J.; Saltsman, J. A. 3rd; Gehart, H.; Torbenson, M. S.; Clevers, H.; LaQuaglia, M. P.; Simon, S. M.
Article Title: Human liver organoids for disease modeling of fibrolamellar carcinoma
Abstract: Fibrolamellar carcinoma (FLC) is a rare, often lethal, liver cancer affecting adolescents and young adults, for which there are no approved therapeutics. The development of therapeutics is hampered by a lack of in vitro models. Organoids have shown utility as a model system for studying many diseases. In this study, tumor tissue and the adjacent non-tumor liver were obtained at the time of surgery. The tissue was dissociated and grown as organoids. We developed 21 patient-derived organoid lines: 12 from metastases, three from the liver tumor and six from adjacent non-tumor liver. These patient-derived FLC organoids recapitulate the histologic morphology, immunohistochemistry, and transcriptome of the patient tumor. Patient-derived FLC organoids were used in a preliminary high-throughput drug screen to show proof of concept for the identification of therapeutics. This model system has the potential to improve our understanding of this rare cancer and holds significant promise for drug testing and development. © 2022 The Author(s)
Keywords: adolescent; liver cell carcinoma; carcinoma, hepatocellular; liver neoplasms; pathology; liver tumor; liver cancer; pediatric cancer; fibrolamellar; humans; human; organoids; organoid
Journal Title: Stem Cell Reports
Volume: 17
Issue: 8
ISSN: 2213-6711
Publisher: Cell Press  
Date Published: 2022-08-09
Start Page: 1874
End Page: 1888
Language: English
DOI: 10.1016/j.stemcr.2022.06.003
PUBMED: 35803261
PROVIDER: scopus
PMCID: PMC9391427
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85135689782&doi=10.1016%2fj.stemcr.2022.06.003&partnerID=40&md5=1ceaa217c59bdaa75dd3f5c452eb08b6
Notes: MSK author Nicole Croteau is listed in the publisher record and PDF as Nicole J.C. Narayan -- Export Date: 1 September 2022 -- Source: Scopus
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MSK Authors
  1. Michael P La Quaglia
    411 La Quaglia
  2. William Joseph Hammond
    13 Hammond
  3. James A Saltsman
    9 Saltsman
  4. Nicole J Croteau
    10 Croteau
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