Optical nanosensor for intracellular and intracranial detection of amyloid-beta Journal Article
| Authors: | Antman-Passig, M.; Wong, E.; Frost, G. R.; Cupo, C.; Shah, J.; Agustinus, A.; Chen, Z.; Mancinelli, C.; Kamel, M.; Li, T.; Jonas, L. A.; Li, Y. M.; Heller, D. A. |
| Article Title: | Optical nanosensor for intracellular and intracranial detection of amyloid-beta |
| Abstract: | Amyloid-beta (A beta) deposition occurs in the early stages of Alzheimer's disease (AD), but the early detection of A beta is a persistent challenge. Herein, we engineered a near-infrared optical nanosensor capable of detecting A beta intracellularly in live cells and intracranially in vivo. The sensor is composed of single-walled carbon nanotubes functionalized with A beta wherein A beta-A beta interactions drive the response. We found that the A beta nanosensors selectively responded to A beta via solvatochromic modulation of the near-infrared emission of the nanotube. The sensor tracked A beta accumulation in live cells and, upon intracranial administration in a genetic model of AD, signaled distinct responses in aged mice. This technology enables the interrogation of molecular mechanisms underlying A beta neurotoxicity in the development of AD in living systems. |
| Keywords: | proteins; fluorescence; peptide; toxicity; recognition; carbon; molecular; alzheimers-disease; a-beta; aggregation; walled carbon nanotubes; cellular uptake; dispersion; nanomaterials; biosensor; neurodegenerative disease; nanocarbon |
| Journal Title: | ACS Nano |
| Volume: | 16 |
| Issue: | 5 |
| ISSN: | 1936-0851 |
| Publisher: | American Chemical Society |
| Date Published: | 2022-05-24 |
| Start Page: | 7269 |
| End Page: | 7283 |
| Language: | English |
| ACCESSION: | WOS:000812148900014 |
| DOI: | 10.1021/acsnano.2c00054 |
| PROVIDER: | wos |
| PUBMED: | 35420796 |
| PMCID: | PMC9710299 |
| Notes: | Article -- Source: Wos |
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