A carbon nanotube reporter of microRNA hybridization events in vivo Journal Article


Authors: Harvey, J. D.; Jena, P. V.; Baker, H. A.; Zerze, G. H.; Williams, R. M.; Galassi, T. V.; Roxbury, D.; Mittal, J.; Heller, D. A.
Article Title: A carbon nanotube reporter of microRNA hybridization events in vivo
Abstract: MicroRNAs and other small oligonucleotides in biofluids are promising disease biomarkers, yet conventional assays require complex processing steps that are unsuitable for point-of-care testing or for implantable or wearable sensors. Single-walled carbon nanotubes are an ideal material for implantable sensors, owing to their emission in the near-infrared spectral region, photostability and exquisite sensitivity. Here, we report an engineered carbon-nanotube-based sensor capable of real-time optical quantification of hybridization events of microRNA and other oligonucleotides. The mechanism of the sensor arises from competitive effects between displacement of both oligonucleotide charge groups and water from the nanotube surface, which result in a solvatochromism-like response. The sensor, which allows for detection via single-molecule sensor elements and for multiplexing by using multiple nanotube chiralities, can monitor toehold-based strand-displacement events, which reverse the sensor response and regenerate the sensor complex. We also show that the sensor functions in whole urine and serum, and can non-invasively measure DNA and microRNA after implantation in live mice.
Keywords: fluorescence; recognition; argonaute2; serum; ovarian-cancer; nucleic-acids; molecular-dynamics; sensors; circulating tumor dna; dna strand displacement
Journal Title: Nature Biomedical Engineering
Volume: 1
Issue: 4
ISSN: 2157-846X
Publisher: Nature Publishing Group  
Date Published: 2017-04-01
Start Page: 0041
Language: English
ACCESSION: WOS:000418855100003
DOI: 10.1038/s41551-017-0041
PROVIDER: wos
PMCID: PMC5568023
PUBMED: 28845337
Notes: Article -- Source: Wos
Altmetric
Citation Impact
MSK Authors
  1. Daniel Alan Heller
    73 Heller
  2. Prakrit Vaibhav Jena
    24 Jena
  3. Thomas Vito Galassi
    13 Galassi
  4. Jackson D Harvey
    13 Harvey
  5. Hanan Baker
    6 Baker