Deploying RNA and DNA with functionalized carbon nanotubes Journal Article
| Authors: | Alidori, S.; Asqiriba, K.; Londero, P.; Bergkvist, M.; Leona, M.; Scheinberg, D. A.; McDevitt, M. R. |
| Article Title: | Deploying RNA and DNA with functionalized carbon nanotubes |
| Abstract: | Carbon nanotubes internalize into cells and are potential molecular platforms for siRNA and DNA delivery. A comprehensive understanding of the identity and stability of ammonium-functionalized carbon nanotube (f-CNT)-based nucleic acid constructs is critical to deploying them in vivo as gene delivery vehicles. This work explored the capability of f-CNT to bind single- and double-strand oligonucleotides by determining the thermodynamics and kinetics of assembly and the stoichiometric composition in aqueous solution. Surprisingly, the binding affinity of f-CNT and short oligonucleotide sequences was in the nanomolar range, kinetics of complexation were extremely rapid, and from one to five sequences were loaded per nanotube platform. Mechanistic evidence for an assembly process that involved electrostatic, hydrogen bonding, and π-stacking bonding interactions was obtained by varying nanotube functionalities, oligonucleotides, and reaction conditions. 31P NMR and spectrophotometric fluorescence emission data described the conditions required to assemble and stably bind a DNA or RNA cargo for delivery in vivo and the amount of oligonucleotide that could be transported. The soluble oligonucleic acid-f-CNT supramolecular assemblies were suitable for use in vivo. Importantly, key evidence in support of an elegant mechanism by which the bound nucleic acid material can be "off-loaded" from the f-CNT was discovered. © 2013 American Chemical Society. |
| Keywords: | gene transfer; rna; binding energy; drug therapy; oligonucleotides; hydrogen bonds; carbon nanotubes; oligonucleotide sequences; bonding interactions; fluorescence emission; functionalized carbon nanotubes; reaction conditions; stoichiometric compositions; supramolecular assemblies; thermodynamics and kinetics |
| Journal Title: | Journal of Physical Chemistry C |
| Volume: | 117 |
| Issue: | 11 |
| ISSN: | 1932-7447 |
| Publisher: | American Chemical Society |
| Date Published: | 2013-03-21 |
| Start Page: | 5982 |
| End Page: | 5992 |
| Language: | English |
| PROVIDER: | scopus |
| PMCID: | PMC3634719 |
| PUBMED: | 23626864 |
| DOI: | 10.1021/jp312416d |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 May 2013" - ":doi 10.1021/jp312416d" - "Source: Scopus" |
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493Scheinberg -
11Alidori -
1
Asqiriba
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