Volumetric interferometric lattice light-sheet imaging Journal Article
| Authors: | Cao, B.; Coelho, S.; Li, J.; Wang, G.; Pertsinidis, A. |
| Article Title: | Volumetric interferometric lattice light-sheet imaging |
| Abstract: | Live cell imaging with high spatiotemporal resolution and high detection sensitivity facilitates the study of the dynamics of cellular structure and function. However, extracting high-resolution 4D (3D space plus time) information from live cells remains challenging, because current methods are slow, require high peak excitation intensities or suffer from high out-of-focus background. Here we present 3D interferometric lattice light-sheet (3D-iLLS) imaging, a technique that requires low excitation light levels and provides high background suppression and substantially improved volumetric resolution by combining 4Pi interferometry with selective plane illumination. We demonstrate that 3D-iLLS has an axial resolution and single-particle localization precision of 100 nm (FWHM) and <10 nm (1σ), respectively. We illustrate the performance of 3D-iLLS in a range of systems: single messenger RNA molecules, nanoscale assemblies of transcription regulators in the nucleus, the microtubule cytoskeleton and mitochondria organelles. The enhanced 4D resolution and increased signal-to-noise ratio of 3D-iLLS will facilitate the analysis of biological processes at the sub-cellular level. © 2021, The Author(s), under exclusive licence to Springer Nature America, Inc. |
| Keywords: | live-cell imaging; rna; high resolution; signal to noise ratio; cellular function; detection sensitivity; interferometry; light sheet; cellular structure; spatio-temporal resolution; volumetrics; crystal lattices; 3d spaces; interferometrics |
| Journal Title: | Nature Biotechnology |
| Volume: | 39 |
| Issue: | 11 |
| ISSN: | 1087-0156 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2021-11-01 |
| Start Page: | 1385 |
| End Page: | 1393 |
| Language: | English |
| DOI: | 10.1038/s41587-021-01042-y |
| PUBMED: | 34635835 |
| PROVIDER: | scopus |
| PMCID: | PMC8595582 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 December 2021 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
-
16Pertsinidis -
6Wang -
6Li -
1Cao
Related MSK Work