Direct-laser writing for subnanometer focusing and single-molecule imaging Journal Article
| Authors: | Coelho, S.; Baek, J.; Walsh, J.; Justin Gooding, J.; Gaus, K. |
| Article Title: | Direct-laser writing for subnanometer focusing and single-molecule imaging |
| Abstract: | Two-photon direct laser writing is an additive fabrication process that utilizes two-photon absorption of tightly focused femtosecond laser pulses to implement spatially controlled polymerization of a liquid-phase photoresist. Two-photon direct laser writing is capable of nanofabricating arbitrary three-dimensional structures with nanometer accuracy. Here, we explore direct laser writing for high-resolution optical microscopy by fabricating unique 3D optical fiducials for single-molecule tracking and 3D single-molecule localization microscopy. By having control over the position and three-dimensional architecture of the fiducials, we improve axial discrimination and demonstrate isotropic subnanometer 3D focusing (<0.8 nm) over tens of micrometers using a standard inverted microscope. We perform 3D single-molecule acquisitions over cellular volumes, unsupervised data acquisition and live-cell single-particle tracking with nanometer accuracy. © 2022, The Author(s). |
| Keywords: | polymerization; absorption; data acquisition; imaging method; accuracy assessment; laser method |
| Journal Title: | Nature Communications |
| Volume: | 13 |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2022-01-01 |
| Start Page: | 647 |
| Language: | English |
| DOI: | 10.1038/s41467-022-28219-6 |
| PUBMED: | 35115532 |
| PROVIDER: | scopus |
| PMCID: | PMC8813935 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 March 2022 -- Source: Scopus |
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