Limiting dilution microculture assay for quantitation of T lymphocytes in bone marrow Conference Paper
| Authors: | Kernan, N. A.; Burns, M. J.; Collins, N. H.; O'Reilly, R. J.; Dupont, B. |
| Title: | Limiting dilution microculture assay for quantitation of T lymphocytes in bone marrow |
| Conference Title: | 10th International Congress of the Transplantation Society |
| Abstract: | This limiting dilution microculture assay is an in vitro quantitative assay for enumerating residual T lymphocytes in T cell-depleted BM. This assay requires only 1 x 106 BM cells. By determining the fraction of negative wells for each cell dose, one can determine the frequency of T lymphocytes in a given sample and thus calculate the absolute number of T lymphocytes in that sample. The sensitivity of the assay was such that 7.5 x 105 T lymphocytes were identified in a sample containing 1.6 x 109 mononuclear cells. |
| Keywords: | methodology; t lymphocyte; bone marrow; in vitro study; phytohemagglutinin; human; priority journal; blood and hemopoietic system |
| Journal Title | Transplantation Proceedings |
| Volume: | 17 |
| Issue: | 1 |
| Conference Dates: | 1984 Aug 26-31 |
| Conference Location: | Minneapolis, MN |
| ISBN: | 0041-1345 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1985-02-01 |
| Start Page: | 437 |
| End Page: | 439 |
| Language: | English |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
