Clonable T lymphocytes in T cell-depleted bone marrow transplants correlate with development of graft-v-host disease Journal Article
| Authors: | Kernan, N. A.; Collins, N. H.; Juliano, L.; Cartagena, T.; Dupont, B.; O'Reilly, R. J. |
| Article Title: | Clonable T lymphocytes in T cell-depleted bone marrow transplants correlate with development of graft-v-host disease |
| Abstract: | Early clinical trials using T lymphocyte-depleted human marrow for transplantation have reported that such grafts reduce, to varying degrees, both the incidence and the severity of graft-v-host disease (GVHD). However, to date, no clear estimates have been made as to what degree of T cell depletion is necessary to prevent GVHD in every case. To address this problem, we used a limiting dilution assay (LDA) to quantitate residual clonable T lymphocytes in human T cell-depleted bone marrow in 31 HLA-identical transplants for leukemia. The number of phytohemagglutinin-interleukin 2-responsive T lymphocytes determined by LDA and expressed as T cell per kilogram recipient weight was found to correlate with the subsequent development of GVHD: no patients who received less than 1 x 105 T cell per kilogram developed GVHD (N = 24). Of the seven patients who received 1 x 105 to 4.4 x 105 T cell per kilogram, four patients developed grade I or II skin GVHD. This study thus provides a quantitative estimate of the number of T lymphocytes necessary to initiate clinically detectable GVHD in an HLA-identical host. |
| Keywords: | human cell; t lymphocyte; bone marrow; graft versus host reaction; bone marrow transplantation; etiology; phytohemagglutinin; human; priority journal; blood and hemopoietic system |
| Journal Title: | Blood |
| Volume: | 68 |
| Issue: | 3 |
| ISSN: | 0006-4971 |
| Publisher: | American Society of Hematology |
| Date Published: | 1986-09-01 |
| Start Page: | 770 |
| End Page: | 773 |
| Language: | English |
| DOI: | 10.1182/blood.v68.3.770.bloodjournal683770 |
| PUBMED: | 3527302 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
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