Characterization of envelope and core structural gene products of HTLV-III with sera from AIDS patients Journal Article
| Authors: | Robey, W. G.; Safai, B.; Oroszlan, S.; Arthur, L. O.; Gonda, M. A.; Gallo, R. C.; Fischinger, P. J. |
| Article Title: | Characterization of envelope and core structural gene products of HTLV-III with sera from AIDS patients |
| Abstract: | The envelope (env) and structural (gag) gene products of human T-cell leukemia (lymphotropic) virus type III were identified by immunoaffinity chromatography, immunoprecipitation, and two-dimensional oligopeptide mapping methods. The env gene specifies a glycosylated polypeptide with a molecular weight of 160,000 (gp160) that is processed to gp120 and smaller gene products. The gag gene specifies two polypeptides of 70,000 and 55,000 molecular weight (p70 and p55), both of which contain p24, the major structural protein of the mature virion. The techniques in this study can be used to define the extent of variability of the env gene product among different virus isolates and may identify the nature and patterns of the humoral immune response that lead to an immunologically protected state. |
| Keywords: | animals; classification; immunoprecipitation; human immunodeficiency virus; acquired immune deficiency syndrome; acquired immunodeficiency syndrome; sarcoma, kaposi; radioisotope; molecular weight; human immunodeficiency virus 1; virus protein; viral proteins; chromatography, affinity; antibodies, viral; virus antibody; pan troglodytes; viral envelope proteins; genes, viral; chromatography; virus envelope; virus characterization; humans; human; priority journal; htlv; deltaretrovirus; blood and hemopoietic system; virus nucleoprotein; oligonucleotide fingerprinting |
| Journal Title: | Science |
| Volume: | 228 |
| Issue: | 4699 |
| ISSN: | 0036-8075 |
| Publisher: | American Association for the Advancement of Science |
| Date Published: | 1985-05-03 |
| Start Page: | 593 |
| End Page: | 595 |
| Language: | English |
| DOI: | 10.1126/science.2984774 |
| PUBMED: | 2984774 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
