Cell surface antigen of human neuroblastomas is related to nuclear antigen of normal cells Journal Article

   


Authors: Rettig, W. J.; Chesa, P. G.; Jennings, M. T.; Spengler, B. A.; Melamed, M. R.; Oettgen, H. F.; Biedler, J. L.; Old, L. J.
Article Title: Cell surface antigen of human neuroblastomas is related to nuclear antigen of normal cells
Abstract: The localization of MC25, an antigen first detected on the surface of human neuroblastoma cells, was determined in cultured cells and tissues. Neuroblastoma cell lines (15/17) express the antigen on the surface and in the cytoplasm (scMC25+), whereas 156/160 cell lines derived from other normal and malignant human cell types are scMC25- cells (nMC25+), presenting a discrete granular pattern. In scMC25+/nMC25- neuroblastoma lines, apparent antigen shifting from the cell surface/cytoplasm to the nucleus accompanies variant formation, which represents a transition in the neuronal differentiation program of these cells. Results of immunohistochemical studies with human tissues parallel the findings with cultured cells. Almost all cell types are scMC25-/nMC25+; basal cells of the epidermis are the only cells constitutively expressing cMC25; and a population of neurons are the only scMC25-/nMC25- cells. Alternative localization of MC25 to different cellular compartments and antigen shifting are reminiscent of the behavior of certain developmentally regulated antigens in Drosophila and Xenopus.
Keywords: cytology; cell line; in vitro study; membrane antigen; antibodies, monoclonal; antigens; antigens, neoplasm; cell culture; neuroblastoma; cell nucleus; nerve cell; antigens, surface; differentiation; nervous system; antigens, nuclear; cell nucleus antigen; nucleoproteins; humans; human; priority journal
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 82
Issue: 20
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1985-10-01
Start Page: 6894
End Page: 6898
Language: English
DOI: 10.1073/pnas.82.20.6894
PUBMED: 3863134
PROVIDER: scopus
PMCID: PMC390794
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022403564&doi=10.1073%2fpnas.82.20.6894&partnerID=40&md5=8dff50620b2d77e31114daaf9eed1943
Notes: Article -- Export Date: 26 October 2021 -- Source: Scopus
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MSK Authors
  1. Herbert F Oettgen
    130 Oettgen
  2. Lloyd J Old
    593 Old
  3. June Biedler
    83 Biedler
  4. Pilar Garin-Chesa
    73 Garin-Chesa
  5. Wolfgang J. Rettig
    85 Rettig
  6. Myron Melamed
    148 Melamed
  7. Barbara Spengler
    44 Spengler
  8. Mark Jennings
    15 Jennings
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